ArticleFrontiers in plant science2015
Metabolic engineering of medium-chain fatty acid biosynthesis in Nicotiana benthamiana plant leaf lipids.
Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 66 citations in OpenAlex.
- FatPlants 2.0: an AI-powered platform integrating plant lipid genes, pathways, and literature.The Plant journal : for cell and molecular biology · 2026Article
- Understanding the dynamic nature of plant lipid anabolic and catabolic metabolism is key to sustainable oilseed engineering.The New phytologist · 2026Review
- Phylogenetic Aspects of Higher Plant Lipid Fatty Acid Profile.International journal of molecular sciences · 2025Review
- Overexpression ofFrontiers in plant science · 2024Article
- Reshaping the 2-Pyrone Synthase Active Site for Chemoselective Biosynthesis of Polyketides.Angewandte Chemie (International ed. in English) · 2023Article
- Improving Coconut Using Modern Breeding Technologies: Challenges and Opportunities.Plants (Basel, Switzerland) · 2022Review
- Article
- The chemotype core collection of genus Nicotiana.The Plant journal : for cell and molecular biology · 2022Article
- Article
- Non-targeted Lipidomics Using a Robust and Reproducible Lipid Separation Using UPLC with Charged Surface Hybrid Technology and High-Resolution Mass Spectrometry.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Acyl-CoA-dependent and acyl-CoA-independent avocado acyltransferases positively influence oleic acid content in nonseed triacylglycerols.Frontiers in plant science · 2022Article
- Is CRISPR/Cas9 a way forward to fast-track genetic improvement in commercial palms? Prospects and limits.Frontiers in plant science · 2022Review
- Article
- Metabolically engineered rice biomass and grain using genes associated with lipid pathway show high level of oil content.Molecular biology reports · 2020Article
- A Versatile High Throughput Screening Platform for Plant Metabolic Engineering Highlights the Major Role ofFrontiers in plant science · 2020Article
- Producing Cyclopropane Fatty Acid in Plant Leafy BiomassFrontiers in plant science · 2020Article
- Comparison of the Substrate Preferences of ω3 Fatty Acid Desaturases for Long Chain Polyunsaturated Fatty Acids.International journal of molecular sciences · 2019Article
- Cellular Organization and Regulation of Plant Glycerolipid Metabolism.Plant & cell physiology · 2019Review
- Identification of Genes Involved in Lipid Biosynthesis through de novo Transcriptome Assembly from Cocos nucifera Developing Endosperm.Plant & cell physiology · 2019Article
- Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Various research groups are investigating the production of oil in non-seed biomass such as leaves. Recently, high levels of oil accumulation have been achieved in plant biomass using a combination of biotechnological approaches which also resulted in significant changes to the fatty acid composition of the leaf oil. In this study, we were interested to determine whether medium-chain fatty acids (MCFA) could be accumulated in leaf oil. MCFA are an ideal feedstock for biodiesel and a range of oleochemical products including lubricants, coatings, and detergents. In this study, we explore the synthesis, accumulation, and glycerolipid head-group distribution of MCFA in leaves of Nicotiana benthamiana after transient transgenic expression of C12:0-, C14:0-, and C16:0-ACP thioesterase genes. We demonstrate that the production of these MCFA in leaf is increased by the co-expression of the WRINKLED1 (WRI1) transcription factor, with the lysophosphatidic acid acyltransferase (LPAAT) from Cocos nucifera being required for the assembly of tri-MCFA TAG species. We also demonstrate that the newly-produced MCFA are incorporated into the triacylglycerol of leaves in which WRI1 + diacylglycerol acyltransferase1 (DGAT1) genes are co-expressed for increased oil accumulation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.