Evidence map›Paper›PMID 25852227›Full record

ArticleCanadian journal of veterinary research = Revue canadienne de recherche veterinaire2015

Identification of Escherichia coli F4ac-binding proteins in porcine milk fat globule membrane.

Predrag Novakovic, Yanyun Y Huang, Betty Lockerbie, Farshid Shahriar, John Kelly, John R Gordon, Dorothy M Middleton, Matthew E Loewen, Beverly A Kidney, Elemir Simko

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in Canadian journal of veterinary research = Revue canadienne de recherche veterinaire, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.0field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

  1. Review
  2. Shiga Toxin-ProducingMicroorganisms · 2022
    Review
  3. Review
  4. Review
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Predrag NovakovicVeterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan (Novakovic, Huang, Lockerbie, Middleton, Kidney, Simko); California Animal Health and Food Safety Laboratory System, University of California, Davis, 105 West Central Avenue, San Bernardino, California, USA (Shahriar); Institute for Biological Sciences, National Research Council Canada, 100 Sussex Drive, Ottawa, Ontario (Kelly); Department of Medicine, College of Medicine, University of Saskatchewan (Gordon); Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan (Loewen).
Yanyun Y HuangVeterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan (Novakovic, Huang, Lockerbie, Middleton, Kidney, Simko); California Animal Health and Food Safety Laboratory System, University of California, Davis, 105 West Central Avenue, San Bernardino, California, USA (Shahriar); Institute for Biological Sciences, National Research Council Canada, 100 Sussex Drive, Ottawa, Ontario (Kelly); Department of Medicine, College of Medicine, University of Saskatchewan (Gordon); Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan (Loewen).
Betty LockerbieVeterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan (Novakovic, Huang, Lockerbie, Middleton, Kidney, Simko); California Animal Health and Food Safety Laboratory System, University of California, Davis, 105 West Central Avenue, San Bernardino, California, USA (Shahriar); Institute for Biological Sciences, National Research Council Canada, 100 Sussex Drive, Ottawa, Ontario (Kelly); Department of Medicine, College of Medicine, University of Saskatchewan (Gordon); Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan (Loewen).
Farshid ShahriarVeterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan (Novakovic, Huang, Lockerbie, Middleton, Kidney, Simko); California Animal Health and Food Safety Laboratory System, University of California, Davis, 105 West Central Avenue, San Bernardino, California, USA (Shahriar); Institute for Biological Sciences, National Research Council Canada, 100 Sussex Drive, Ottawa, Ontario (Kelly); Department of Medicine, College of Medicine, University of Saskatchewan (Gordon); Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan (Loewen).
John KellyVeterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan (Novakovic, Huang, Lockerbie, Middleton, Kidney, Simko); California Animal Health and Food Safety Laboratory System, University of California, Davis, 105 West Central Avenue, San Bernardino, California, USA (Shahriar); Institute for Biological Sciences, National Research Council Canada, 100 Sussex Drive, Ottawa, Ontario (Kelly); Department of Medicine, College of Medicine, University of Saskatchewan (Gordon); Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan (Loewen).
John R GordonVeterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan (Novakovic, Huang, Lockerbie, Middleton, Kidney, Simko); California Animal Health and Food Safety Laboratory System, University of California, Davis, 105 West Central Avenue, San Bernardino, California, USA (Shahriar); Institute for Biological Sciences, National Research Council Canada, 100 Sussex Drive, Ottawa, Ontario (Kelly); Department of Medicine, College of Medicine, University of Saskatchewan (Gordon); Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan (Loewen).
Dorothy M MiddletonVeterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan (Novakovic, Huang, Lockerbie, Middleton, Kidney, Simko); California Animal Health and Food Safety Laboratory System, University of California, Davis, 105 West Central Avenue, San Bernardino, California, USA (Shahriar); Institute for Biological Sciences, National Research Council Canada, 100 Sussex Drive, Ottawa, Ontario (Kelly); Department of Medicine, College of Medicine, University of Saskatchewan (Gordon); Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan (Loewen).
Matthew E LoewenVeterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan (Novakovic, Huang, Lockerbie, Middleton, Kidney, Simko); California Animal Health and Food Safety Laboratory System, University of California, Davis, 105 West Central Avenue, San Bernardino, California, USA (Shahriar); Institute for Biological Sciences, National Research Council Canada, 100 Sussex Drive, Ottawa, Ontario (Kelly); Department of Medicine, College of Medicine, University of Saskatchewan (Gordon); Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan (Loewen).
Beverly A KidneyVeterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan (Novakovic, Huang, Lockerbie, Middleton, Kidney, Simko); California Animal Health and Food Safety Laboratory System, University of California, Davis, 105 West Central Avenue, San Bernardino, California, USA (Shahriar); Institute for Biological Sciences, National Research Council Canada, 100 Sussex Drive, Ottawa, Ontario (Kelly); Department of Medicine, College of Medicine, University of Saskatchewan (Gordon); Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan (Loewen).
Elemir SimkoVeterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan (Novakovic, Huang, Lockerbie, Middleton, Kidney, Simko); California Animal Health and Food Safety Laboratory System, University of California, Davis, 105 West Central Avenue, San Bernardino, California, USA (Shahriar); Institute for Biological Sciences, National Research Council Canada, 100 Sussex Drive, Ottawa, Ontario (Kelly); Department of Medicine, College of Medicine, University of Saskatchewan (Gordon); Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan (Loewen).
Saskatchewan Research Council (Canada) · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

F4ac-positive enterotoxigenic Escherichia coli (ETEC) must attach to the intestinal mucosa to cause diarrhea in piglets. Prevention of bacterial attachment to the intestinal mucosa is the most effective defense against ETEC-induced diarrhea. Porcine milk fat globule membranes (MFGM) were shown to be able to inhibit attachment of ETEC to the intestinal brush border; however, the specific components of porcine MFGM that inhibited attachment of ETEC to enterocytes were not identified. Accordingly, the purpose of this study was to identify F4ac-binding MFGM proteins by overlay Western blot and affinity chromatography. The proteome of porcine MFGM was characterized and the following F4ac-binding proteins were detected by overlay Western blot and affinity chromatography: lactadherin, butyrophilin, adipophilin, acyl-CoA synthetase 3, and fatty acid-binding protein 3. The biological function of these proteins was not investigated but it is possible that their interaction with F4ac fimbria interferes with bacterial attachment and colonization.

Indexed as

AnimalsBacterial AdhesionBlotting, WesternChromatography, AffinityEnterotoxigenic Escherichia coliEscherichia coli ProteinsFemaleGlycolipidsGlycoproteinsLipid DropletsProtein BindingSwineEscherichia coli ProteinsGlycolipidsGlycoproteinsmilk fat globule

Identifiers

PMID25852227
PMCPMC4365703
OpenAlexW312268407

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.