ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2015
Design and validation of a qPCR assay for accurate detection and initial serogrouping of Legionella pneumophila in clinical specimens by the ESCMID Study Group for Legionella Infections (ESGLI).
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Impact of urine concentration and automatic interpretation on sensitivity and specificity of Legionella BinaxNOWEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Can Digital PCR Enhance the Detection ofPathogens (Basel, Switzerland) · 2026Article
- MixedInfectious disease reports · 2026Article
- Preliminary report: The most considerable outbreak of Legionnaires' disease in France in the last two decades, Albertville, September 2025.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2025Article
- Comparison of Phenotypic and Whole-Genome Sequencing-Derived Antimicrobial Resistance Profiles ofAntibiotics (Basel, Switzerland) · 2025Article
- Temporal and Spatial Survey on the Abundance of Amoebae and Bacteria in an Estuary and the Role of Environmental Parameters.Environmental microbiology reports · 2025Article
- Characterisation ofMicroorganisms · 2024Article
- Legionnaires' Disease: Update on Diagnosis and Treatment.Infectious diseases and therapy · 2022Review
- Use of Fourier-Transform Infrared Spectroscopy With IR Biotyper® System forFrontiers in microbiology · 2022Article
- Case Report: First Report of FatalFrontiers in medicine · 2022Article
- Article
- Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2021Article
- Optimising the case-crossover design for use in shared exposure settings.Epidemiology and infection · 2020Article
- Short-term associations between Legionnaires' disease incidence and meteorological variables in Belgium, 2011-2019.Epidemiology and infection · 2020Article
- Improvement of Legionnaires' disease diagnosis using real-time PCR assay: a retrospective analysis, Italy, 2010 to 2015.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2018Article
- Comparison of in situ sequence type analysis of Legionella pneumophila in respiratory tract secretions and environmental samples of a hospital in East Jerusalem.Epidemiology and infection · 2018Article
- Spatial structuring of a Legionella pneumophila population within the water system of a large occupational building.Microbial genomics · 2018Article
- On-filter direct amplification of Legionella pneumophila for rapid assessment of its abundance and viability.Water research · 2017Article
Corrections and comments
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Authors and funding
10 authors at 6 institutions in 6 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Prompt detection of Legionella pneumophila is essential for rapid investigation of legionellosis. Furthermore, as the majority of L. pneumophila infections are caused by serogroup 1 (sg1) strains, rapid identification of such strains can be critical in both routine and outbreak scenarios. The ESCMID Study Group for Legionella Infections (ESGLI) was established in 2012 and immediately identified as a priority the validation of a reliable, easy to perform and interpret, cost-effective qPCR assay to standardise the detection of L. pneumophila DNA amongst members. A novel L. pneumophila assay targeting the mip gene was designed and combined with previously published methodologies amplifying the sg1 marker (wzm) and the green fluorescent protein gene (gfp) internal process control. The resulting triplex assay was validated internationally on the three qPCR platforms used by the majority of European Legionella reference laboratories: ABI 7500 (Life Technologies), LightCycler 480 Instrument II (Roche) and Rotor-Gene Q (Qiagen). Clinical and EQA specimens were tested together with a large panel of strains (251 in total) to validate the assay. The assay proved to be 100% specific for L. pneumophila and sg1 DNA both in silico and in vitro. Efficiency values for mip and wzm assays ranged between 91.97 and 97.69%. Limit of detection values estimated with 95% confidence were adopted for mip and wzm assays on all three qPCR platforms. Inhibition was not observed. This study describes a robust assay that could be widely implemented to standardise the molecular detection of L. pneumophila among ESGLI laboratories and beyond.
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