Evidence map›Paper›PMID 25851648›Full record

ArticleEMBO reports2015

Wnt directs the endosomal flux of LDL-derived cholesterol and lipid droplet homeostasis.

Cameron C Scott, Stefania Vossio, Fabrizio Vacca, Berend Snijder, Jorge Larios, Olivier Schaad, Nicolas Guex, Dmitry Kuznetsov, Olivier Martin, Marc Chambon and 3 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in EMBO reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
2.4field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 51 citations in OpenAlex.

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  17. Life in the lumen: The multivesicular endosome.Traffic (Copenhagen, Denmark) · 2020
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 6 institutions in 1 country.

Cameron C ScottDepartment of Biochemistry, University of Geneva, Geneva, Switzerland.
Stefania VossioDepartment of Biochemistry, University of Geneva, Geneva, Switzerland.
Fabrizio VaccaDepartment of Biochemistry, University of Geneva, Geneva, Switzerland.
Berend SnijderFaculty of Sciences, Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.
Jorge LariosDepartment of Biochemistry, University of Geneva, Geneva, Switzerland.
Olivier SchaadDepartment of Biochemistry, University of Geneva, Geneva, Switzerland.
Nicolas GuexVital-IT Group, Swiss Institute of Bioinformatics, University of Lausanne, Lausanne, Switzerland.
Dmitry KuznetsovVital-IT Group, Swiss Institute of Bioinformatics, University of Lausanne, Lausanne, Switzerland.
Olivier MartinVital-IT Group, Swiss Institute of Bioinformatics, University of Lausanne, Lausanne, Switzerland.
Marc ChambonBiomolecular Screening Facility, SV-PTECH-PTCB, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland.
Gerardo TurcattiBiomolecular Screening Facility, SV-PTECH-PTCB, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland.
Lucas PelkmansFaculty of Sciences, Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.
Jean GruenbergDepartment of Biochemistry, University of Geneva, Geneva, Switzerland jean.gruenberg@unige.ch.
University of Geneva · CHÉcole Polytechnique Fédérale de Lausanne · CHSIB Swiss Institute of Bioinformatics · CHLife Science Zurich · CHUniversity of Lausanne · CHUniversity of Zurich · CH

Funding

Canadian Institutes of Health Research
6 · The paper itself

Abstract

The Wnt pathway, which controls crucial steps of the development and differentiation programs, has been proposed to influence lipid storage and homeostasis. In this paper, using an unbiased strategy based on high-content genome-wide RNAi screens that monitored lipid distribution and amounts, we find that Wnt3a regulates cellular cholesterol. We show that Wnt3a stimulates the production of lipid droplets and that this stimulation strictly depends on endocytosed, LDL-derived cholesterol and on functional early and late endosomes. We also show that Wnt signaling itself controls cholesterol endocytosis and flux along the endosomal pathway, which in turn modulates cellular lipid homeostasis. These results underscore the importance of endosome functions for LD formation and reveal a previously unknown regulatory mechanism of the cellular programs controlling lipid storage and endosome transport under the control of Wnt signaling.

Indexed as

Wnt Signaling PathwayAnimalsCell LineCholesterol, LDLEndocytosisEndosomesEpithelial CellsGene Expression ProfilingHeLa CellsHigh-Throughput Screening AssaysHomeostasisHumansL CellsLipid DropletsMiceOleic AcidCholesterol, LDLOleic AcidWnt3A ProteinWNT3A protein, humanWnt3a protein, mousecanonical Wnt signalingendosomesfunctional genomicsLDL‐derived cholesterollipid droplets

Identifiers

PMID25851648
PMCPMC4467858
OpenAlexW2117858538

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.