ArticleMolecular biology of the cell2015
Asymmetric formation of coated pits on dorsal and ventral surfaces at the leading edges of motile cells and on protrusions of immobile cells.
Article in Molecular biology of the cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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Who cites it
23 citing papers in PubMed, 40 citations in OpenAlex.
- Spatial N-glycan rearrangement on αNature communications · 2025Article
- Spatial Regulation of Endocytosis and Adhesion Formation Governs Breast Cancer Cell Migration Under Confinement.Bioengineering (Basel, Switzerland) · 2025Article
- Growth factor-triggered de-sialylation controls glycolipid-lectin-driven endocytosis.Nature cell biology · 2025Article
- Mechano-inhibition of endocytosis sensitizes cancer cells to Fas-induced Apoptosis.Cell death & disease · 2024Article
- The Plasma Membrane and Mechanoregulation in Cells.ACS omega · 2024Review
- Endocytosis at extremes: Formation and internalization of giant clathrin-coated pits under elevated membrane tension.Frontiers in molecular biosciences · 2022Article
- Clathrin-coated structures support 3D directed migration through local force transmission.Science advances · 2021Article
- Dynamic interplay between cell membrane tension and clathrin-mediated endocytosis.Biology of the cell · 2021Review
- YAP1 nuclear efflux and transcriptional reprograming follow membrane diminution upon VSV-G-induced cell fusion.Nature communications · 2021Article
- Article
- An internally eGFP-tagged α-adaptin is a fully functional and improved fiduciary marker for clathrin-coated pit dynamics.Traffic (Copenhagen, Denmark) · 2020Article
- 4D cell biology: big data image analytics and lattice light-sheet imaging reveal dynamics of clathrin-mediated endocytosis in stem cell-derived intestinal organoids.Molecular biology of the cell · 2018Article
- Article
- Regulation of Clathrin-Mediated Endocytosis.Annual review of biochemistry · 2018Review
- Membrane mechanics govern spatiotemporal heterogeneity of endocytic clathrin coat dynamics.Molecular biology of the cell · 2017Article
- Mechanoregulation of clathrin-mediated endocytosis.Journal of cell science · 2017Article
- Recruitment dynamics of ESCRT-III and Vps4 to endosomes and implications for reverse membrane budding.eLife · 2017Article
- Membrane dynamics of dividing cells imaged by lattice light-sheet microscopy.Molecular biology of the cell · 2016Article
- The ubiquitin-proteasome system regulates focal adhesions at the leading edge of migrating cells.eLife · 2016Article
- Deciphering dynamics of clathrin-mediated endocytosis in a living organism.The Journal of cell biology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Clathrin/AP2-coated vesicles are the principal endocytic carriers originating at the plasma membrane. In the experiments reported here, we used spinning-disk confocal and lattice light-sheet microscopy to study the assembly dynamics of coated pits on the dorsal and ventral membranes of migrating U373 glioblastoma cells stably expressing AP2 tagged with enhanced green fluorescence (AP2-EGFP) and on lateral protrusions from immobile SUM159 breast carcinoma cells, gene-edited to express AP2-EGFP. On U373 cells, coated pits initiated on the dorsal membrane at the front of the lamellipodium and at the approximate boundary between the lamellipodium and lamella and continued to grow as they were swept back toward the cell body; coated pits were absent from the corresponding ventral membrane. We observed a similar dorsal/ventral asymmetry on membrane protrusions from SUM159 cells. Stationary coated pits formed and budded on the remainder of the dorsal and ventral surfaces of both types of cells. These observations support a previously proposed model that invokes net membrane deposition at the leading edge due to an imbalance between the endocytic and exocytic membrane flow at the front of a migrating cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.