Evidence map›Paper›PMID 25851001›Full record

ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2015

Physical mapping of chromosome 4J of Thinopyrum bessarabicum using gamma radiation-induced aberrations.

Jing Pu, Qing Wang, Yuefeng Shen, Lifang Zhuang, Chenxu Li, Mengfa Tan, Tongde Bie, Chenggen Chu, Zengjun Qi

Abstract read
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Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

  1. Introgression of stem rust resistance gene Sr68 from Thinopyrum junceum into wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025
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  4. Development and characterization of novelMolecular breeding : new strategies in plant improvement · 2021
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  12. Characterisation of Thinopyrum bessarabicum chromosomes through genome-wide introgressions into wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018
    Article
  13. Physical mapping of DNA markers linked to stem rust resistance gene Sr47 in durum wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2017
    Article
  14. Mapping of novel powdery mildew resistance gene(s) from Agropyron cristatum chromosome 2P.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2017
    Article
  15. Article
  16. Physical mapping of Agropyron cristatum chromosome 6P using deletion lines in common wheat background.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2016
    Article
  17. Article
4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Jing PuState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University/JCIC-MCP, Nanjing, 210095, China.
Qing Wang
Yuefeng Shen
Lifang Zhuang
Chenxu Li
Mengfa Tan
Tongde Bie
Chenggen Chu
Zengjun Qi

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

key messageGamma radiation induced a series of structural aberrations involving Thinopyrum bessarabicum chromosome 4J. The aberrations allowed for deletion mapping of 101 4J-specific markers and fine mapping of blue-grained gene BaThb. Irradiation can induce translocations and deletions to assist physically locating genes and markers on chromosomes. In this study, a 12-Gy dosage of (60)Co-γ was applied to pollen and eggs of a wheat (Triticum aestivum) landrace Chinese Spring (CS)-Thinopyrum bessarabicum chromosome 4J disomic addition line (DA4J), and the gametes from irradiated plants were fertilized with normal CS eggs or pollen to produce M1 seeds. Based on genomic in situ hybridization analysis of 261 M1 plants, we identified 74 lines carrying structural aberrations involving chromosome 4J with the higher aberration rate in treated pollen (31.2 %) than in the treated eggs (21.3 %). We further identified 43 (53.8 %) lines with structural aberrations on chromosome 4J by analyzing another 80 M1 plants with 74 4J-specific markers, indicating that combining molecular and cytological methods was more efficient for detecting chromosome aberrations. Marker analysis thus was performed prior to cytogenetic identification on M2-M4 seeds to detect chromosome structural aberrations. Sixty-eight M3 lines with structural aberrations on chromosome 4J and six previously obtained chromosome 4J alien lines were then analyzed using 101 chromosome 4J-specific markers. After combining marker results with chromosome aberrations in each line, chromosome 4J was physically divided into 24 segmental blocks with 7 in the short arm and 17 in the long arm. The blue-grained gene BaThb was further mapped into the region corresponding to block 4JL-11. The chromosome aberrations and the physical map developed in this research provide useful stocks and tools for introgression of genes on chromosome 4J into wheat.

Indexed as

Gamma RaysGene DeletionPhysical Chromosome MappingChromosome AberrationsChromosomes, PlantGenes, PlantGenetic MarkersOvulePoaceaePollenTriticumGenetic Markers

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