ArticleBMC plant biology2015
DOF AFFECTING GERMINATION 2 is a positive regulator of light-mediated seed germination and is repressed by DOF AFFECTING GERMINATION 1.
Article in BMC plant biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 55 citations in OpenAlex.
- Dof Transcription Factors as Integrative Regulatory Hubs Linking Carbon-Nitrogen Metabolism, Development, and Environmental Signaling in Plants.Plant, cell & environment · 2026Review
- Genome-wide association study of pre-harvest sprouting resistance and grain color in common wheat (Triticum aestivum L.).BMC plant biology · 2025Article
- Identification of theBiology · 2025Article
- The transcription factors DOF4.6 and XND1 jointly regulate root hydraulics and drought responses in Arabidopsis.The Plant cell · 2025Article
- Genome-Wide Identification and Functional Characterization of the Dof Family inInternational journal of molecular sciences · 2025Article
- Establishment of single-cell transcriptional states during seed germination.Nature plants · 2024Article
- Genome-Wide Identification of theInternational journal of molecular sciences · 2024Article
- The Role ofPlants (Basel, Switzerland) · 2024Article
- DOF gene family expansion and diversification.Genetics and molecular biology · 2024Article
- Genome-Wide Identification and Expression Analysis of thePlants (Basel, Switzerland) · 2023Article
- DOF transcription factors: Specific regulators of plant biological processes.Frontiers in plant science · 2023Review
- Genome-Wide Identification and Analysis ofGenes · 2022Article
- Network of the transcriptome and metabolomics reveals a novel regulation of drought resistance during germination in wheat.Annals of botany · 2022Article
- Effects of the Rhizosphere FungusInternational journal of molecular sciences · 2022Article
- Expression profiling of the Dof gene family under abiotic stresses in spinach.Scientific reports · 2021Article
- Genome-WidePlants (Basel, Switzerland) · 2021Article
- Unravelling the Complex Interplay of Transcription Factors Orchestrating Seed Oil Content inInternational journal of molecular sciences · 2021Article
- Genome-wide analysis ofPeerJ · 2021Article
- The DOF Transcription Factors in Seed and Seedling Development.Plants (Basel, Switzerland) · 2020Review
- Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe transcription factor DOF AFFECTING GERMINATION1 (DAG1) is a repressor of the light-mediated seed germination process. DAG1 acts downstream PHYTOCHROME INTERACTING FACTOR3-LIKE 5 (PIL5), the master repressor, and negatively regulates gibberellin biosynthesis by directly repressing the biosynthetic gene AtGA3ox1. The Dof protein DOF AFFECTING GERMINATION (DAG2) shares a high degree of aminoacidic identity with DAG1. While DAG1 inactivation considerably increases the germination capability of seeds, the dag2 mutant has seeds with a germination potential substantially lower than the wild-type, indicating that these factors may play opposite roles in seed germination.
resultsWe show here that DAG2 expression is positively regulated by environmental factors triggering germination, whereas its expression is repressed by PIL5 and DAG1; by Chromatin Immuno Precipitation (ChIP) analysis we prove that DAG1 directly regulates DAG2. In addition, we show that Red light significantly reduces germination of dag2 mutant seeds.
conclusionsIn agreement with the seed germination phenotype of the dag2 mutant previously published, the present data prove that DAG2 is a positive regulator of the light-mediated seed germination process, and particularly reveal that this protein plays its main role downstream of PIL5 and DAG1 in the phytochrome B (phyB)-mediated pathway.
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