ArticleBMC plant biology2015
Powerful regulatory systems and post-transcriptional gene silencing resist increases in cellulose content in cell walls of barley.
Article in BMC plant biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 45 citations in OpenAlex.
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- CRISPR/Cas9-mediated P-CR domain-specific engineering of CESA4 heterodimerization capacity alters cell wall architecture and improves saccharification efficiency in poplar.Plant biotechnology journal · 2022Article
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- Multi-locus genome-wide association mapping for spike-related traits in bread wheat (Triticum aestivum L.).BMC genomics · 2021Article
- Engineering of Bioenergy Crops: Dominant Genetic Approaches to Improve Polysaccharide Properties and Composition in Biomass.Frontiers in plant science · 2020Review
- Increase in Cell Wall Thickening and Biomass Production by Overexpression ofFrontiers in plant science · 2020Article
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- Origin, evolution, and divergence of plant class C GH9 endoglucanases.BMC evolutionary biology · 2018Article
- Three AtCesA6-like members enhance biomass production by distinctively promoting cell growth in Arabidopsis.Plant biotechnology journal · 2018Article
- Secondary Wall Regulating NACs Differentially Bind at the Promoter at aFrontiers in plant science · 2018Article
- A Transcriptomic Analysis of Xylan Mutants Does Not Support the Existence of a Secondary Cell Wall Integrity System in Arabidopsis.Frontiers in plant science · 2018Article
- Functional Analysis of Cellulose SynthaseFrontiers in plant science · 2018Article
- Genetic engineering of grass cell wall polysaccharides for biorefining.Plant biotechnology journal · 2017Review
- Morphology, Carbohydrate Distribution, Gene Expression, and Enzymatic Activities Related to Cell Wall Hydrolysis in Four Barley Varieties during Simulated Malting.Frontiers in plant science · 2017Article
- Emerging Technologies for the Production of Renewable Liquid Transport Fuels from Biomass Sources Enriched in Plant Cell Walls.Frontiers in plant science · 2016Review
- Genetics and physiology of cell wall polysaccharides in the model C4 grass, Setaria viridis spp.BMC plant biology · 2015Article
- Cell wall composition and digestibility alterations in Brachypodium distachyon achieved through reduced expression of the UDP-arabinopyranose mutase.Frontiers in plant science · 2015Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe ability to increase cellulose content and improve the stem strength of cereals could have beneficial applications in stem lodging and producing crops with higher cellulose content for biofuel feedstocks. Here, such potential is explored in the commercially important crop barley through the manipulation of cellulose synthase genes (CesA).
resultsBarley plants transformed with primary cell wall (PCW) and secondary cell wall (SCW) barley cellulose synthase (HvCesA) cDNAs driven by the CaMV 35S promoter, were analysed for growth and morphology, transcript levels, cellulose content, stem strength, tissue morphology and crystalline cellulose distribution. Transcript levels of the PCW HvCesA transgenes were much lower than expected and silencing of both the endogenous CesA genes and introduced transgenes was often observed. These plants showed no aberrant phenotypes. Although attempts to over-express the SCW HvCesA genes also resulted in silencing of the transgenes and endogenous SCW HvCesA genes, aberrant phenotypes were sometimes observed. These included brittle nodes and, with the 35S:HvCesA4 construct, a more severe dwarfing phenotype, where xylem cells were irregular in shape and partially collapsed. Reductions in cellulose content were also observed in the dwarf plants and transmission electron microscopy showed a significant decrease in cell wall thickness. However, there were no increases in overall crystalline cellulose content or stem strength in the CesA over-expression transgenic plants, despite the use of a powerful constitutive promoter.
conclusionsThe results indicate that the cellulose biosynthetic pathway is tightly regulated, that individual CesA proteins may play different roles in the synthase complex, and that the sensitivity to CesA gene manipulation observed here suggests that in planta engineering of cellulose levels is likely to require more sophisticated strategies.
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