ArticlePloS one2015
Evaluation and validation of housekeeping genes as reference for gene expression studies in pigeonpea (Cajanus cajan) under drought stress conditions.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41 citing papers in PubMed, 80 citations in OpenAlex.
- Transcriptional regulation reveals potent drought tolerance mechanisms in contrasting genotypes of Cajanus cajan (L.) Millspaugh.BMC plant biology · 2025Article
- Interspecies predictions of growth traits from quantitative transcriptome data acquired during fruit development.Journal of experimental botany · 2025Article
- Genome-wide identification and expression profiling of growth‑regulating factor (GRF) and GRF‑interacting factor (GIF) gene families in chickpea and pigeonpea.Scientific reports · 2024Article
- Understanding the role of P-type ATPases in regulating pollen fertility and development in pigeonpea.Molecular genetics and genomics : MGG · 2024Article
- Drought and heat stress: insights into tolerance mechanisms and breeding strategies for pigeonpea improvement.Planta · 2024Review
- Screening and validation of reference genes in Dracaena cochinchinensis using quantitative real-time PCR.Scientific reports · 2024Article
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- Genomic characterization of ZIP genes in pigeonpea (Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Identification and validation of reference genes for qRT-PCR based studies in horse gram (Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Global gene expression analysis of pigeonpea with male sterility conditioned by AThe plant genome · 2021Article
- Genome-Wide Identification, Genomic Organization, and Characterization of Potassium Transport-Related Genes inPlants (Basel, Switzerland) · 2021Article
- Induction of moisture stress tolerance by3 Biotech · 2021Article
- Reference genes for quantitative real-time PCR normalization of Cenostigma pyramidale roots under salt stress and mycorrhizal association.Genetics and molecular biology · 2021Article
- Genomics-assisted breeding for pigeonpea improvement.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Review
- Molecular Analysis of Disease-Responsive Genes Revealing the Resistance Potential Against Fusarium Wilt (Frontiers in genetics · 2020Article
- Analysis of reference genes stability and histidine kinase expression under cold stress in Cordyceps militaris.PloS one · 2020Article
- Identification of stable reference genes for qPCR studies in common wheat (Plant methods · 2020Article
- Comparative transcriptome analyses provide novel insights into the differential response of Pigeonpea (Cajanus cajan L.) and its wild relative (Cajanus platycarpus (Benth.) Maesen) to herbivory by Helicoverpa armigera (Hübner).Plant molecular biology · 2019Article
- Understanding the Role of the WRKY Gene Family under Stress Conditions in Pigeonpea (Plants (Basel, Switzerland) · 2019Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene expression analysis using quantitative real-time PCR (qRT-PCR) is a very sensitive technique and its sensitivity depends on the stable performance of reference gene(s) used in the study. A number of housekeeping genes have been used in various expression studies in many crops however, their expression were found to be inconsistent under different stress conditions. As a result, species specific housekeeping genes have been recommended for different expression studies in several crop species. However, such specific housekeeping genes have not been reported in the case of pigeonpea (Cajanus cajan) despite the fact that genome sequence has become available for the crop. To identify the stable housekeeping genes in pigeonpea for expression analysis under drought stress conditions, the relative expression variations of 10 commonly used housekeeping genes (EF1α, UBQ10, GAPDH, 18SrRNA, 25SrRNA, TUB6, ACT1, IF4α, UBC and HSP90) were studied on root, stem and leaves tissues of Asha (ICPL 87119). Three statistical algorithms geNorm, NormFinder and BestKeeper were used to define the stability of candidate genes. geNorm analysis identified IF4α and TUB6 as the most stable housekeeping genes however, NormFinder analysis determined IF4α and HSP90 as the most stable housekeeping genes under drought stress conditions. Subsequently validation of the identified candidate genes was undertaken in qRT-PCR based gene expression analysis of uspA gene which plays an important role for drought stress conditions in pigeonpea. The relative quantification of the uspA gene varied according to the internal controls (stable and least stable genes), thus highlighting the importance of the choice of as well as validation of internal controls in such experiments. The identified stable and validated housekeeping genes will facilitate gene expression studies in pigeonpea especially under drought stress conditions.
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