Evidence map›Paper›PMID 25849636›Full record

ArticleNature methods2015

Massively parallel delivery of large cargo into mammalian cells with light pulses.

Yi-Chien Wu, Ting-Hsiang Wu, Daniel L Clemens, Bai-Yu Lee, Ximiao Wen, Marcus A Horwitz, Michael A Teitell, Pei-Yu Chiou

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.

0numbers the graph read from it
0cells of the map it votes in
53citing papers in PubMed
22.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

53 citing papers in PubMed, 174 citations in OpenAlex.

  1. Article
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  14. Article
  15. Advanced tools and methods for single-cell surgery.Microsystems & nanoengineering · 2022
    Review
  16. Review
  17. Micromotor-based localized electroporation and gene transfection of mammalian cells.Proceedings of the National Academy of Sciences of the United States of America · 2021
    Article
  18. Microfluidic and Nanofluidic Intracellular Delivery.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021
    Review
  19. Article
  20. Electrochemical fabrication of TiOMaterials chemistry and physics · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Yi-Chien WuDepartment of Mechanical and Aerospace Engineering, University of California at Los Angeles, Los Angeles, California, USA.
Ting-Hsiang Wu1] Department of Mechanical and Aerospace Engineering, University of California at Los Angeles, Los Angeles, California, USA. [2] Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California, USA.
Daniel L ClemensDivision of Infectious Diseases, Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California, USA.
Bai-Yu LeeDivision of Infectious Diseases, Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California, USA.
Ximiao WenDepartment of Mechanical and Aerospace Engineering, University of California at Los Angeles, Los Angeles, California, USA.
Marcus A HorwitzDivision of Infectious Diseases, Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California, USA.
Michael A Teitell1] Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California, USA. [2] Department of Bioengineering, University of California at Los Angeles, Los Angeles, California, USA. [3] California NanoSystems Institute (CNSI), University of California at Los Angeles, Los Angeles, California, USA.
Pei-Yu Chiou1] Department of Mechanical and Aerospace Engineering, University of California at Los Angeles, Los Angeles, California, USA. [2] Department of Bioengineering, University of California at Los Angeles, Los Angeles, California, USA. [3] California NanoSystems Institute (CNSI), University of California at Los Angeles, Los Angeles, California, USA.
University of California, Los Angeles · US

Funding

Vaccines and Diagnostics TrialsU54AI065359 · NIAID · UNIVERSITY OF CALIFORNIA-IRVINE · PI BARBOUR, ALAN G. · 2005 to 2013
$88.8M
Reverse Mitochondrial Genetics Enabled by BlastR01GM114188 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CHIOU, PEI-YU, TEITELL, MICHAEL A · 2015 to 2018
$1.9M
Microfluidics-Integrated Photothermal Nanoblade for High-Throughput Large Cargo DR21EB014456 · NIBIB · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CHIOU, PEI-YU, TEITELL, MICHAEL A · 2012 to 2013
$387k
NIAID NIH HHS AI065359NIAID NIH HHS U54 AI065359NIBIB NIH HHS EB014456NIBIB NIH HHS R21 EB014456NIGMS NIH HHS GM114188NIGMS NIH HHS R01 GM114188
6 · The paper itself

Abstract

We report a high-throughput platform for delivering large cargo elements into 100,000 cells in 1 min. Our biophotonic laser-assisted surgery tool (BLAST) generates an array of microcavitation bubbles that explode in response to laser pulsing, forming pores in adjacent cell membranes through which cargo is gently driven by pressurized flow. The platform delivers large items including bacteria, enzymes, antibodies and nanoparticles into diverse cell types with high efficiency and cell viability. We used this platform to explore the intracellular lifestyle of Francisella novicida and discovered that the iglC gene is unexpectedly required for intracellular replication even after phagosome escape into the cell cytosol.

Indexed as

LasersMicrobubblesAnimalsCell LineFrancisellaGene Expression Regulation, BacterialHumans

Identifiers

PMID25849636
PMCPMC5082232
OpenAlexW2075547024

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.