ArticlePloS one2015
Loss-of-function analysis reveals distinct requirements of the translation initiation factors eIF4E, eIF4E-3, eIF4G and eIF4G2 in Drosophila spermatogenesis.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 57 citations in OpenAlex.
- Review
- Comparative single-nucleus transcriptomics reveals asymmetric evolution of the Drosophila male and female germlines.PLoS biology · 2026Article
- Non-gonadal PIWIL1/Aubergine drives regenerative and tumorigenic stem cell proliferation and tumorigenesis in the intestine.Cell reports · 2026Article
- EIF5A Couples Translational Control With Transcriptional Reprogramming Through Chromocenter Reorganization During Spermiogenesis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Remodeling of Germ Cell mRNPs for Translational Control.Biology · 2025Review
- Dichotomous transcriptome divergence at the cell-type level between thebioRxiv : the preprint server for biology · 2025Article
- Signals from the niche promote distinct modes of translation initiation to control stem cell differentiation and renewal in the Drosophila testis.PLoS biology · 2025Article
- C8ORF88: A Novel eIF4E-Binding Protein.Genes · 2023Article
- Editing melon eIF4E associates with virus resistance and male sterility.Plant biotechnology journal · 2022Article
- Cyst stem cell lineage eIF5 non-autonomously prevents testicular germ cell tumor formation via eIF1A/eIF2γ-mediated pre-initiation complex.Stem cell research & therapy · 2022Article
- Ribosome heterogeneity in Drosophila melanogaster gonads through paralog-switching.Nucleic acids research · 2022Article
- Review
- Unconventional translation initiation factor EIF2A is required for Drosophila spermatogenesis.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- Ribosome heterogeneity and specialization in development.Wiley interdisciplinary reviews. RNA · 2021Review
- The Dynamic Regulation of mRNA Translation and Ribosome Biogenesis During Germ Cell Development and Reproductive Aging.Frontiers in cell and developmental biology · 2021Review
- Distinct roles of two eIF4E isoforms in the germline ofJournal of cell science · 2020Article
- Article
- Defining the expression of piRNA and transposable elements inLife science alliance · 2019Article
- A ribosomal protein S5 isoform is essential for oogenesis and interacts with distinct RNAs in Drosophila melanogaster.Scientific reports · 2019Article
- Specialized eRpL22 paralogue-specific ribosomes regulate specific mRNA translation in spermatogenesis inMolecular biology of the cell · 2019Article
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2 authors at 1 institution in 1 country.
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Abstract
In eukaryotes, post-transcriptional regulation of gene expression has a key role in many cellular and developmental processes. Spermatogenesis involves a complex developmental program that includes changes in cell cycle dynamics and dramatic cellular remodeling. Translational control is critical for spermatogenesis in Drosophila as many mRNAs synthesized in the spermatocytes are translated only much later during spermatid differentiation. Testes-specific translation initiation factors eIF4E-3 and eIF4G2 are essential specifically for male fertility. However, details of their roles during different stages of spermatogenesis are unknown, and the role of canonical translation initiation factors in spermatogenesis remains unexplored. In this study, we addressed the functional role of eIF4E-1, eIF4E-3, eIF4G and eIF4G2 in testes development and formation of mature sperm. Using the UAS-Gal4 system and RNA interference, we systematically knocked down these four genes in different stages of germ cell development, and in the somatic cells. Our results show that eIF4E-1 function in early germ cells and the surrounding somatic cells is critical for spermatogenesis. Both eIF4E-1 and eIF4E-3 are required in spermatocytes for chromosome condensation and cytokinesis during the meiotic stages. Interestingly, we find that eIF4G knockdown did not affect male fertility while eIF4G2 has distinct functions during spermatogenesis; it is required in early germ cells for proper meiotic divisions and spermatid elongation while its abrogation in spermatocytes caused meiotic arrest. Double knockdown of eIF4G and eIF4G2 shows that these proteins act redundantly during the early stages of spermatogenesis. Taken together, our analysis reveals spatio-temporal roles of the canonical and testes-specific translation initiation factors in coordinating developmental programs during spermatogenesis.
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