ArticleBreast cancer research : BCR2015
Mouse mammary stem cells express prognostic markers for triple-negative breast cancer.
Article in Breast cancer research : BCR, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 40 citations in OpenAlex.
- Activation of PI3K and deletion of p53 in keratin 15-expressing mouse mammary cells induces tumor heterogeneity and plasticity modeling metaplastic breast cancer.The Journal of pathology · 2025Article
- Droplet-based proteomics reveals CD36 as a marker for progenitors in mammary basal epithelium.Cell reports methods · 2024Article
- Mechanosensitive hormone signaling promotes mammary progenitor expansion and breast cancer risk.Cell stem cell · 2024Article
- Aberrant activation of p53/p66Shc-mInsc axis increases asymmetric divisions and attenuates proliferation of aged mammary stem cells.Cell death and differentiation · 2022Article
- Defining mammary basal cell transcriptional states using single-cell RNA-sequencing.Scientific reports · 2022Article
- Multiple Metastases of the Liver and Lung After Breast-Conserving Surgery for Ductal CarcinomaFrontiers in oncology · 2022Review
- Nfatc1's Role in Mammary Epithelial Morphogenesis and Basal Stem/progenitor Cell Self-renewal.Journal of mammary gland biology and neoplasia · 2021Article
- Article
- Reproductive history determines Erbb2 locus amplification, WNT signalling and tumour phenotype in a murine breast cancer model.Disease models & mechanisms · 2021Article
- From structural resilience to cell specification - Intermediate filaments as regulators of cell fate.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Review
- Dissecting the Rat Mammary Gland: Isolation, Characterization, and Culture of Purified Mammary Epithelial Cells and Fibroblasts.Bio-protocol · 2020Article
- Rapid activation of epithelial-mesenchymal transition drives PARP inhibitor resistance inOncotarget · 2019Article
- p53 Loss in Breast Cancer Leads to Myc Activation, Increased Cell Plasticity, and Expression of a Mitotic Signature with Prognostic Value.Cell reports · 2019Article
- Gene Expression Profiling for Diagnosis of Triple-Negative Breast Cancer: A Multicenter, Retrospective Cohort Study.Frontiers in oncology · 2019Article
- Overexpression of miR-489 derails mammary hierarchy structure and inhibits HER2/neu-induced tumorigenesis.Oncogene · 2019Article
- Serially transplantable mammary epithelial cells express the Thy-1 antigen.Breast cancer research : BCR · 2018Article
- MELK as a potential target to control cell proliferation in triple-negative breast cancer MDA-MB-231 cells.Oncology letters · 2018Article
- Single-cell RNA-Seq reveals cell heterogeneity and hierarchy within mouse mammary epithelia.The Journal of biological chemistry · 2018Article
- Breast cancer stem cell markers CD44 and ALDH1A1 in serum: distribution and prognostic value in patients with primary breast cancer.Journal of Cancer · 2018Article
- Circulating cancer stem cell markers in breast carcinomas: a systematic review protocol.Systematic reviews · 2017Article
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Authors and funding
11 authors at 5 institutions in 3 countries.
Funding
Abstract
introductionTriple-negative breast cancer (TNBC) is a heterogeneous group of tumours in which chemotherapy, the current mainstay of systemic treatment, is often initially beneficial but with a high risk of relapse and metastasis. There is currently no means of predicting which TNBC will relapse. We tested the hypothesis that the biological properties of normal stem cells are re-activated in tumour metastasis and that, therefore, the activation of normal mammary stem cell-associated gene sets in primary TNBC would be highly prognostic for relapse and metastasis.
methodsMammary basal stem and myoepithelial cells were isolated by flow cytometry and tested in low-dose transplant assays. Gene expression microarrays were used to establish expression profiles of the stem and myoepithelial populations; these were compared to each other and to our previously established mammary epithelial gene expression profiles. Stem cell genes were classified by Gene Ontology (GO) analysis and the expression of a subset analysed in the stem cell population at single cell resolution. Activation of stem cell genes was interrogated across different breast cancer cohorts and within specific subtypes and tested for clinical prognostic power.
resultsA set of 323 genes was identified that was expressed significantly more highly in the purified basal stem cells compared to all other cells of the mammary epithelium. A total of 109 out of 323 genes had been associated with stem cell features in at least one other study in addition to our own, providing further support for their involvement in the biology of this cell type. GO analysis demonstrated an enrichment of these genes for an association with cell migration, cytoskeletal regulation and tissue morphogenesis, consistent with a role in invasion and metastasis. Single cell resolution analysis showed that individual cells co-expressed both epithelial- and mesenchymal-associated genes/proteins. Most strikingly, we demonstrated that strong activity of this stem cell gene set in TNBCs identified those tumours most likely to rapidly progress to metastasis.
conclusionsOur findings support the hypothesis that the biological properties of normal stem cells are drivers of metastasis and that these properties can be used to stratify patients with a highly heterogeneous disease such as TNBC.
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