ArticleActa crystallographica. Section F, Structural biology communications2015
Large-volume protein crystal growth for neutron macromolecular crystallography.
Article in Acta crystallographica. Section F, Structural biology communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 44 citations in OpenAlex.
- Flow as a Control Strategy to Promote Biomolecule Nucleation.Advances in biochemical engineering/biotechnology · 2026Review
- Resistive Pulse Sensing of Pre-Nucleation Activities during Single-Entity Lysozyme Crystallization on Single Nanopipettes.Sensors and actuators reports · 2025Article
- Counter-diffusion studies of human transthyretin: the growth of high-quality crystals for X-ray and neutron crystallography.Journal of applied crystallography · 2025Article
- Improved joint X-ray and neutron refinement procedure in Phenix.Acta crystallographica. Section D, Structural biology · 2023Article
- Equilibration of precipitants in a counter-diffusion apparatus for protein crystallization.Journal of applied crystallography · 2023Article
- Perfect Crystals: microgravity capillary counterdiffusion crystallization of human manganese superoxide dismutase for neutron crystallography.NPJ microgravity · 2023Article
- The mechanisms of catalysis and ligand binding for the SARS-CoV-2 NSP3 macrodomain from neutron and x-ray diffraction at room temperature.Science advances · 2022Article
- Microgravity crystallization of perdeuterated tryptophan synthase for neutron diffraction.NPJ microgravity · 2022Article
- Leveraging Spaceflight to Advance Cardiovascular Research on Earth.Circulation research · 2022Review
- The mechanisms of catalysis and ligand binding for the SARS-CoV-2 NSP3 macrodomain from neutron and X-ray diffraction at room temperature.bioRxiv : the preprint server for biology · 2022Article
- Metalloprotein catalysis: structural and mechanistic insights into oxidoreductases from neutron protein crystallography.Acta crystallographica. Section D, Structural biology · 2021Review
- Semi-empirical model to estimate ideal conditions for the growth of large protein crystals.Acta crystallographica. Section D, Structural biology · 2020Article
- Perdeuteration, large crystal growth and neutron data collection of Leishmania mexicana triose-phosphate isomerase E65Q variant.Acta crystallographica. Section F, Structural biology communications · 2019Article
- Evaluation of models determined by neutron diffraction and proposed improvements to their validation and deposition.Acta crystallographica. Section D, Structural biology · 2018Review
- Protein crystallization and initial neutron diffraction studies of the photosystem II subunit PsbO.Acta crystallographica. Section F, Structural biology communications · 2017Article
- Review
- Neutron and high-resolution room-temperature X-ray data collection from crystallized lytic polysaccharide monooxygenase.Acta crystallographica. Section F, Structural biology communications · 2015Article
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neutron macromolecular crystallography (NMC) is the prevailing method for the accurate determination of the positions of H atoms in macromolecules. As neutron sources are becoming more available to general users, finding means to optimize the growth of protein crystals to sizes suitable for NMC is extremely important. Historically, much has been learned about growing crystals for X-ray diffraction. However, owing to new-generation synchrotron X-ray facilities and sensitive detectors, protein crystal sizes as small as in the nano-range have become adequate for structure determination, lessening the necessity to grow large crystals. Here, some of the approaches, techniques and considerations for the growth of crystals to significant dimensions that are now relevant to NMC are revisited. These include experimental strategies utilizing solubility diagrams, ripening effects, classical crystallization techniques, microgravity and theoretical considerations.
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