ArticlePloS one2015
Ecdysteroids regulate the levels of Molt-Inhibiting Hormone (MIH) expression in the blue crab, Callinectes sapidus.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 85 citations in OpenAlex.
- Inducing bursicon expression using 20-hydroxyecdysone (20E) increased immune response in Macrobrachium rosenbergii against Aeromonas hydrophila.Biology open · 2025Article
- Physiology of Ovarian Development in Crustaceans: Interactions Among Hormones, Nutrients, and Environmental Factors From Integrated Perspectives.Aquaculture nutrition · 2025Review
- Identifying the genes involved in the egg-carrying ovigerous hair development of the female blue crab Callinectes sapidus: transcriptomic and genomic expression analyses.BMC genomics · 2023Article
- Comparative Study on Growth Index and Nutritional Quality of Female Chinese Mitten CrabAquaculture nutrition · 2023Article
- Dietary cholesterol promotes growth and ecdysone signalling pathway by modulating cholesterol transport in swimming crabs (Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2022Article
- 17β-Estradiol (E2) may be involved in the mode of crustacean female sex hormone (CFSH) action in the blue crab,Frontiers in endocrinology · 2022Article
- Article
- Chromosome-level genome assembly of the blue crab, Callinectes sapidus.G3 (Bethesda, Md.) · 2021Article
- Pharmacological Activities forFoods (Basel, Switzerland) · 2021Review
- Article
- A Crab Is Not a Fish: Unique Aspects of the Crustacean Endocrine System and Considerations for Endocrine Toxicology.Frontiers in endocrinology · 2021Review
- Reconstruction ofFrontiers in genetics · 2020Article
- The Crustacean Hyperglycemic Hormone Superfamily: Progress Made in the Past Decade.Frontiers in endocrinology · 2020Review
- Effects of Organotins on Crustaceans: Update and Perspectives.Frontiers in endocrinology · 2018Review
- Ecdysone Receptor Agonism Leading to Lethal Molting Disruption in Arthropods: Review and Adverse Outcome Pathway Development.Environmental science & technology · 2017Review
- Transcriptomic variation of eyestalk reveals the genes and biological processes associated with molting in Portunus trituberculatus.PloS one · 2017Article
- Vitellogenin and vitellogenin receptor gene expression and 20-hydroxyecdysone concentration in Macrobrachium rosenbergii exposed to chlordecone.Environmental science and pollution research international · 2016Article
- Comparative analyses of olfactory systems in terrestrial crabs (Brachyura): evidence for aerial olfaction?PeerJ · 2015Article
- The Involvement of Hemocyte Prophenoloxidase in the Shell-Hardening Process of the Blue Crab, Callinectes sapidus.PloS one · 2015Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Arthropod molt is coordinated through the interplay between ecdysteroids and neuropeptide hormones. In crustaceans, changes in the activity of Y-organs during the molt cycle have been regulated by molt-inhibiting hormone (MIH) and crustacean hyperglycemic hormone (CHH). Little has been known of the mode of direct effects of ecdysteroids on the levels of MIH and CHH in the eyestalk ganglia during the molt cycle. This study focused on a putative feedback of ecdysteroids on the expression levels of MIH transcripts using in vitro incubation study with ecdysteroids and in vivo RNAi in the blue crab, Callinectes sapidus. Our results show a specific expression of ecdysone receptor (EcR) in which EcR1 is the major isoform in eyestalk ganglia. The initial elevation of MIH expression at the early premolt stages is replicated by in vitro incubations of eyestalk ganglia with ecdysteroids that mimic the intrinsic conditions of D0 stage: the concentration (75 ng/ml) and composition (ponasterone A and 20-hydroxyecdysone at a 3:1 (w:w) ratio). Additionally, multiple injections of EcR1-dsRNA reduce MIH expression by 67%, compared to the controls. Our data provide evidence on a putative feedback mechanism of hormonal regulation during molting cycle, specifically how the molt cycle is repeated during the life cycle of crustaceans. The elevated concentrations of ecdysteroids at early premolt stage may act positively on the levels of MIH expression in the eyestalk ganglia. Subsequently, the increased MIH titers in the hemolymph at postmolt would inhibit the synthesis and release of ecdysteroids by Y-organs, resulting in re-setting the subsequent molt cycle.
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