ArticleBMC plant biology2015
Cell wall modification in tobacco by differential targeting of recombinant endoglucanase from Trichoderma reesei.
Article in BMC plant biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
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- Acidothermus cellulolyticus E1 endoglucanase expressed in planta undergoes extensive hydroxyproline-O-glycosylation and exhibits enhanced impact on biomass digestibility.Plant cell reports · 2024Article
- Targeted genome editing of plants and plant cells for biomanufacturing.Transgenic research · 2021Review
- Temperature Differentially Influences the Capacity ofFrontiers in plant science · 2021Article
- Engineering of Bioenergy Crops: Dominant Genetic Approaches to Improve Polysaccharide Properties and Composition in Biomass.Frontiers in plant science · 2020Review
- Small, Smaller, Nano: New Applications for Potato Virus X in Nanotechnology.Frontiers in plant science · 2019Review
- Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility.Biotechnology for biofuels · 2018Article
- Plant Molecular Farming - Integration and Exploitation of Side Streams to Achieve Sustainable Biomanufacturing.Frontiers in plant science · 2018Review
- Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase.Scientific reports · 2017Article
- The TcEG1 beetle (Biotechnology for biofuels · 2017Article
- Article
- Strategies for the production of cell wall-deconstructing enzymes in lignocellulosic biomass and their utilization for biofuel production.Plant biotechnology journal · 2016Review
- Transcriptome-wide sequencing provides insights into geocarpy in peanut (Arachis hypogaea L.).Plant biotechnology journal · 2016Article
- Transgenic Plant-Produced Hydrolytic Enzymes and the Potential of Insect Gut-Derived Hydrolases for Biofuels.Frontiers in plant science · 2016Review
- RNA-Seq and iTRAQ Reveal the Dwarfing Mechanism of Dwarf Polish Wheat (Triticum polonicum L.).International journal of biological sciences · 2016Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe development of transgenic plants as a production platform for biomass-degrading enzymes is a promising tool for an economically feasible allocation of enzymes processing lignocellulose. Previous research has already identified a major limitation of in planta production such as interference with the structure and integrity of the plant cell wall resulting in a negative influence on plant growth and development.
resultsHere, we describe the in planta expression of endoglucanase TrCel5A from the mesophilic fungus Trichoderma reesei with differential intracellular targeting and evaluate its impact on the tobacco cell wall composition. Targeting of the enzyme to the apoplast leads to distinct changes in cell polysaccharides such as glucose level in the matrix polysaccharides (MPS). These effects are combined with severe changes in plant development. Retention of TrCel5A in the endoplasmic reticulum (ER) could avoid visible effects on plant growth under the chosen conditions, but exhibits changes in the composition of the MPS.
conclusionsThese results give new insights into the complex interaction of heterologous cellulase expression with cell wall development and it outlines novel promising strategies to engineer plant cell walls for improved biomass processing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.