ArticlePloS one2015
Recombinant hamster oviductin is biologically active and exerts positive effects on sperm functions and sperm-oocyte binding.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- Mechanistic understanding of heat stress in cattle reproduction toward pharmacological strategies: review.Animal bioscience · 2026Article
- Functional Methods for Studying Sperm-Zona Pellucida Interaction in Mammals.Methods and protocols · 2025Review
- Functional characterization and phenotyping of RAB2A and Lactadherin/MFGE8 as boar sperm zona pellucida binding proteins.Frontiers in cell and developmental biology · 2025Article
- Molecular Evolution of theAnimals : an open access journal from MDPI · 2024Article
- The sperm-interacting proteome in the bovine isthmus and ampulla during the periovulatory period.Journal of animal science and biotechnology · 2023Article
- Pre-Treatment of Swine Oviductal Epithelial Cells with Progesterone Increases the Sperm Fertilizing Ability in an IVF Model.Animals : an open access journal from MDPI · 2022Article
- Spatiotemporal profiling of the bovine oviduct fluid proteome around the time of ovulation.Scientific reports · 2022Article
- The role of oviduct-specific glycoprotein (OVGP1) in modulating biological functions of gametes and embryos.Histochemistry and cell biology · 2022Review
- A Comparative View on the Oviductal Environment during the Periconception Period.Biomolecules · 2020Review
- Sperm Proteome after Interaction with Reproductive Fluids in Porcine: From the Ejaculation to the Fertilization Site.International journal of molecular sciences · 2020Article
- Human OVGP1 enhances tyrosine phosphorylation of proteins in the fibrous sheath involving AKAP3 and increases sperm-zona binding.Journal of assisted reproduction and genetics · 2019Article
- Genes Encoding Mammalian Oviductal Proteins Involved in Fertilization are Subjected to Gene Death and Positive Selection.Journal of molecular evolution · 2018Article
- Hormonal regulation of oviductal glycoprotein 1 (OVGP1; MUC9) in the rhesus macaque cervix.Journal of medical primatology · 2018Article
- The Trithorax group protein dMLL3/4 instructs the assembly of the zygotic genome at fertilization.EMBO reports · 2018Article
- Review: The epic journey of sperm through the female reproductive tract.Animal : an international journal of animal bioscience · 2018Review
- Extra-oviductal expression of oviductal glycoprotein 1 in mouse: Detection in testis, epididymis and ovary.Journal of biosciences · 2017Article
- Effect of recombinant and native buffalo OVGP1 on sperm functions and in vitro embryo development: a comparative study.Journal of animal science and biotechnology · 2017Article
- The C-terminal region of OVGP1 remodels the zona pellucida and modifies fertility parameters.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Studies carried out in several mammalian species suggest that oviductin, also known as oviduct-specific glycoprotein or OVGP1, plays a key role in sperm capacitation, fertilization, and development of early embryos. In the present study, we used recombinant DNA technology to produce, for the first time, recombinant hamster OVGP1 (rHamOVGP1) in human embryonic kidney 293 (HEK293) cells. rHamOVGP1 secreted in the culture medium was purified by affinity chromatography. The resulting protein migrated as a poly-dispersed band of 160-350 kDa on SDS-PAGE corresponding to the molecular mass of the native HamOVGP1. Subsequent mass spectrometric analysis of the purified rHamOVGP1 confirmed its identity as HamOVGP1. Immunocytochemistry demonstrated binding of rHamOVGP1 to the mid-piece and head of hamster sperm and to the zona pellucida (ZP) of ovarian oocytes. In vitro functional experiments showed that addition of rHamOVGP1 in the capacitation medium further enhanced tyrosine phosphorylation of two sperm proteins of approximately 75 kDa and 83 kDa in a time-dependent manner. After 3 hours of incubation in the presence of rHamOVGP1, a significant increase in acrosome reaction was measured. Pretreatment of either sperm or oocyte with 20 μg/ml of rHamOVGP1 prior to sperm-egg binding assay significantly increased the number of sperm bound to the ZP. Addition of rHamOVGP1 in the medium during sperm-egg binding with either oocyte or sperm pretreated with rHamOVGP1 also saw an increase in the number of sperm bound to ZP. In all experimental conditions, the effect of rHamOVGP1 on sperm-oocyte binding was negated by the addition of monoclonal anti-HamOVGP1 antibody. The successful production and purification of a biologically active rHamOVGP1 will allow further exploration of the function of this glycoprotein in reproductive function.
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