ArticlePloS one2015
Methylene blue protects astrocytes against glucose oxygen deprivation by improving cellular respiration.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Hexokinase as a Central Hub in Neurodegeneration: From Metabolic Dysfunction to Therapeutic Innovation.Aging and disease · 2025Review
- Mito Stress Assay of PBMCs With Seahorse XFe96 Flux Analyzer and Comparison of Poly-D-Lysine and Poly-L-Lysine for Cell Affinity.Bio-protocol · 2025Article
- Oxidized Carbon Nanoparticles Enhance Cellular Energetics With Application to Injured Brain.Advanced healthcare materials · 2025Article
- Methylene blue at recommended concentrations alters metabolism in early zebrafish development.Communications biology · 2025Article
- Estradiol and 3β-diol protect female cortical astrocytes by regulating connexin 43 Gap Junctions.Molecular and cellular endocrinology · 2023Article
- Metabolic Heterogeneity of Cerebral Cortical and Cerebellar Astrocytes.Life (Basel, Switzerland) · 2023Article
- Therapeutics of Alzheimer's Disease: Recent Developments.Antioxidants (Basel, Switzerland) · 2022Review
- Methylene blue ameliorates brain edema in rats with experimental ischemic stroke via inhibiting aquaporin 4 expression.Acta pharmacologica Sinica · 2021Article
- Modulation of astrocyte phenotype in response to T-cell interaction.Journal of neuroimmunology · 2021Article
- Effect of Methylene Blue and PI3K-Akt Pathway Inhibitors on the Neurovascular System after Chronic Cerebral Hypoperfusion in Rats.Journal of molecular neuroscience : MN · 2020Article
- Cholesterol sulfate alters astrocyte metabolism and provides protection against oxidative stress.Brain research · 2019Article
- A novel serum free primary astrocyte culture method that mimic quiescent astrocyte phenotype.Journal of neuroscience methods · 2019Article
- Assay for Assessing Mitochondrial Function in iPSC-Derived Neural Stem Cells and Dopaminergic Neurons.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Article
- Alternative mitochondrial electron transfer for the treatment of neurodegenerative diseases and cancers: Methylene blue connects the dots.Progress in neurobiology · 2017Review
- Neuroprotective and Functional Improvement Effects of Methylene Blue in Global Cerebral Ischemia.Molecular neurobiology · 2016Article
- Tau-Centric Targets and Drugs in Clinical Development for the Treatment of Alzheimer's Disease.BioMed research international · 2016Review
- Combined activation of the energy and cellular-defense pathways may explain the potent anti-senescence activity of methylene blue.Redox biology · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Astrocytes outnumber neurons and serve many metabolic and trophic functions in the mammalian brain. Preserving astrocytes is critical for normal brain function as well as for protecting the brain against various insults. Our previous studies have indicated that methylene blue (MB) functions as an alternative electron carrier and enhances brain metabolism. In addition, MB has been shown to be protective against neurodegeneration and brain injury. In the current study, we investigated the protective role of MB in astrocytes. Cell viability assays showed that MB treatment significantly protected primary astrocytes from oxygen-glucose deprivation (OGD) & reoxygenation induced cell death. We also studied the effect of MB on cellular oxygen and glucose metabolism in primary astrocytes following OGD-reoxygenation injury. MB treatment significantly increased cellular oxygen consumption, glucose uptake and ATP production in primary astrocytes. In conclusion our study demonstrated that MB protects astrocytes against OGD-reoxygenation injury by improving astrocyte cellular respiration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.