Evidence map›Paper›PMID 25848953›Full record

ArticleBiomacromolecules2015

Structure-Function Assessment of Mannosylated Poly(β-amino esters) upon Targeted Antigen Presenting Cell Gene Delivery.

Charles H Jones, Mingfu Chen, Akhila Gollakota, Anitha Ravikrishnan, Guojian Zhang, Sharon Lin, Myles Tan, Chong Cheng, Haiqing Lin, Blaine A Pfeifer

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomacromolecules, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Charles H JonesDepartment of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, New York, United States.
Mingfu ChenDepartment of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, New York, United States.
Akhila GollakotaDepartment of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, New York, United States.
Anitha RavikrishnanDepartment of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, New York, United States.
Guojian ZhangDepartment of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, New York, United States.
Sharon LinDepartment of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, New York, United States.
Myles TanDepartment of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, New York, United States.
Chong ChengDepartment of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, New York, United States.
Haiqing LinDepartment of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, New York, United States.
Blaine A PfeiferDepartment of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, New York, United States.

Funding

A Hybrid Biomaterial-biological Vector for Antigen Presenting Cell Gene DeliveryR03AI088485 · NIAID · TUFTS UNIVERSITY MEDFORD · PI PFEIFER, BLAINE A. · 2010 to 2011
$145k
NIAID NIH HHS AI088485NIAID NIH HHS R03 AI088485
6 · The paper itself

Abstract

Antigen presenting cell (APC) gene delivery is a promising avenue for modulating immunological outcomes toward a desired state. Recently, our group developed a delivery methodology to elicit targeted and elevated levels of APC-mediated gene delivery. During these initial studies, we observed APC-specific structure-function relationships with the vectors used during gene delivery that differ from current non-APC cell lines, thus, emphasizing a need to re-evaluate vector-associated parameters in the context of APC gene transfer. Thus, we describe the synthesis and characterization of a second-generation mannosylated poly(β-amino ester) library stratified by molecular weight. To better understand the APC-specific structure-function relationships governing polymeric gene delivery, the library was systematically characterized by (1) polymer molecular weight, (2) relative mannose content, (3) polyplex biophysical properties, and (4) gene delivery efficacy. In this library, polymers with the lowest molecular weight and highest relative mannose content possessed gene delivery transfection efficiencies as good as or better than commercial controls. Among this group, the most effective polymers formed the smallest polymer-plasmid DNA complexes (∼300 nm) with moderate charge densities (<10 mV). This convergence in polymer structure and polyplex biophysical properties suggests a unique mode of action and provides a framework within which future APC-targeting polymers can be designed.

Indexed as

Genetic TherapyGene Transfer TechniquesAntigen-Presenting CellsDNAHumansMannosePlasmidsPolymersStructure-Activity RelationshipDNAMannosepoly(beta-amino ester)Polymers

Identifiers

PMID25848953
PMCPMC9898688

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.