ArticleJournal of the American Chemical Society2015
A "Smart" ¹²⁸Xe NMR Biosensor for pH-Dependent Cell Labeling.
Article in Journal of the American Chemical Society, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Hyperpolarized Xenon-129 Chemical Exchange Saturation Transfer (HyperCEST) Molecular Imaging: Achievements and Future Challenges.International journal of molecular sciences · 2024Review
- Opportunities and challenges with hyperpolarized bioresponsive probes for functional imaging using magnetic resonance.Nature chemistry · 2023Review
- Counteranions at Peripheral Sites Tune Guest Affinity for a Protonated Hemicryptophane.The Journal of organic chemistry · 2022Article
- Ultrasensitive molecular building block for biothiol NMR detection at picomolar concentrations.iScience · 2021Article
- Article
- Fast Ion-Chelate Dissociation Rate forJournal of the American Chemical Society · 2021Article
- Versatile non-luminescent color palette based on guest exchange dynamics in paramagnetic cavitands.Nature communications · 2021Article
- Monomeric Cryptophane with Record-High Xe Affinity Gives Insights into Aggregation-Dependent Sensing.Analytical chemistry · 2021Article
- Mapping of Absolute Host Concentration and Exchange Kinetics of Xenon Hyper-CEST MRI Agents.Pharmaceuticals (Basel, Switzerland) · 2021Article
- Cryptophane-xenon complexes forRSC advances · 2021Article
- Functionalization of Polyethyleneimine with Hollow Cyclotriveratrylene and Its Subsequent Supramolecular Interaction with Doxorubicin.Molecules (Basel, Switzerland) · 2020Article
- Molecular Sensing with Host Systems for HyperpolarizedMolecules (Basel, Switzerland) · 2020Review
- Article
- Paramagnetic Organocobalt Capsule Revealing Xenon Host-Guest Chemistry.Inorganic chemistry · 2020Article
- Paramagnetic Shifts and Guest Exchange Kinetics in CoInorganic chemistry · 2020Article
- High Xe density, high photon flux, stopped-flow spin-exchange optical pumping: Simulations versus experiments.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2020Article
- Directly Functionalized Cucurbit[7]uril as a Biosensor for the Selective Detection of Protein Interactions byChemistry (Weinheim an der Bergstrasse, Germany) · 2019Article
- Cryptophane Nanoscale Assemblies ExpandAnalytical chemistry · 2018Article
- Hyperpolarized Amino Acid Derivatives as Multivalent Magnetic Resonance pH Sensor Molecules.Sensors (Basel, Switzerland) · 2018Article
- Cyclodextrin-Based Pseudorotaxanes: Easily Conjugatable Scaffolds for Synthesizing Hyperpolarized Xenon-129 Magnetic Resonance Imaging Agents.ACS omega · 2018Article
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Authors and funding
3 authors.
Funding
Abstract
Here we present a "smart" xenon-129 NMR biosensor that undergoes a peptide conformational change and labels cells in acidic environments. To a cryptophane host molecule with high Xe affinity, we conjugated a 30mer EALA-repeat peptide that is α-helical at pH 5.5 and disordered at pH 7.5. The (129)Xe NMR chemical shift at room temperature was strongly pH-dependent (Δδ = 3.4 ppm): δ = 64.2 ppm at pH 7.5 vs δ = 67.6 ppm at pH 5.5, where Trp(peptide)-cryptophane interactions were evidenced by Trp fluorescence quenching. Using hyper-CEST NMR, we probed peptidocryptophane detection limits at low-picomolar (10(-11) M) concentration, which compares favorably to other NMR pH reporters at 10(-2)-10(-3) M. Finally, in biosensor-HeLa cell solutions, peptide-cell membrane insertion at pH 5.5 generated a 13.4 ppm downfield cryptophane-(129)Xe NMR chemical shift relative to pH 7.5 studies. This highlights new uses for (129)Xe as an ultrasensitive probe of peptide structure and function, along with potential applications for pH-dependent cell labeling in cancer diagnosis and treatment.
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