ArticleACS synthetic biology2015
Rationally Designed MicroRNA-Based Genetic Classifiers Target Specific Neurons in the Brain.
Article in ACS synthetic biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Cell type-specific enhancers regulate IL-22 expression in innate and adaptive type 3 lymphoid cells.Nature communications · 2026Article
- Human Synthetic Biology and Programmable Gene Regulation Control.Annual review of genomics and human genetics · 2025Review
- Novel strategy to target glioblastoma-initiating cells using a braintropic adeno-associated virus carrying a miR-dependent genome-editing system.British journal of cancer · 2025Article
- Article
- CaMKIIα-Positive Interneurons Identified via a microRNA-Based Viral Gene Targeting Strategy.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Viral vectors for neuronal cell type-specific visualization and manipulations.Current opinion in neurobiology · 2020Review
- A MicroRNA-Based Gene-Targeting Tool for Virally Labeling Interneurons in the Rodent Cortex.Cell reports · 2018Article
- A mixed antagonistic/synergistic miRNA repression model enables accurate predictions of multi-input miRNA sensor activity.Nature communications · 2018Article
- MicroRNA-Regulated Gene Delivery Systems for Research and Therapeutic Purposes.Molecules (Basel, Switzerland) · 2018Review
- Computing Mathematical Functions using DNA via Fractional Coding.Scientific reports · 2018Article
- Model-guided combinatorial optimization of complex synthetic gene networks.Molecular systems biology · 2016Article
- Cell-Specific Targeting of Genetically Encoded Tools for Neuroscience.Annual review of genetics · 2016Review
- Strategies for targeting primate neural circuits with viral vectors.Journal of neurophysiology · 2016Review
- MicroRNA-regulated viral vectors for gene therapy.World journal of experimental medicine · 2016Review
- An integrative approach for analyzing hundreds of neurons in task performing mice using wide-field calcium imaging.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Targeting transgene expression to specific cell types in vivo has proven instrumental in characterizing the functional role of defined cell populations. Genetic classifiers, synthetic transgene constructs designed to restrict expression to particular classes of cells, commonly rely on transcriptional promoters to define cellular specificity. However, the large size of many natural promoters complicates their use in viral vectors, an important mode of transgene delivery in the brain and in human gene therapy. Here, we expanded upon an emerging classifier platform, orthogonal to promoter-based strategies, that exploits endogenous microRNA regulation to target gene expression. Such classifiers have been extensively explored in other tissues; however, their use in the nervous system has thus far been limited to targeting gene expression between neurons and supporting cells. Here, we tested the possibility of using combinatory microRNA regulation to specify gene targeting between neuronal subtypes, and successfully targeted inhibitory cells in the neocortex. These classifiers demonstrate the feasibility of designing a new generation of microRNA-based neuron-type- and brain-region-specific gene expression targeting neurotechnologies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.