Evidence map›Paper›PMID 25848745›Full record

ArticleeLife2015

In vivo targeting of de novo DNA methylation by histone modifications in yeast and mouse.

Marco Morselli, William A Pastor, Barbara Montanini, Kevin Nee, Roberto Ferrari, Kai Fu, Giancarlo Bonora, Liudmilla Rubbi, Amander T Clark, Simone Ottonello and 2 more

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in eLife, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 107 papers.

0numbers the graph read from it
0cells of the map it votes in
107citing papers in PubMed
8.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

107 citing papers in PubMed, 172 citations in OpenAlex.

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  11. A Modular and Customizable CRISPR/Cas Toolkit for Epigenome Editing of Cis-regulatory Modules.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
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47 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Marco MorselliDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States.
William A PastorDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States.
Barbara MontaniniBiochemistry and Molecular Biology Unit, Department of Life Sciences, Laboratory of Functional Genomics and Protein Engineering, Parma, Italy.
Kevin NeeDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States.
Roberto FerrariDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States.
Kai FuDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States.
Giancarlo BonoraDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States.
Liudmilla RubbiDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States.
Amander T ClarkDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States.
Simone OttonelloBiochemistry and Molecular Biology Unit, Department of Life Sciences, Laboratory of Functional Genomics and Protein Engineering, Parma, Italy.
Steven E JacobsenDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States.
Matteo PellegriniDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States.
University of California, Los Angeles · USBroad Center · US

Funding

Understanding Epigenetic Remodeling in Primordial Germ CellsR01HD058047 · NICHD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CLARK, AMANDER · 2009 to 2023
$5.6M
Genetics of DNA methylation patterning of arabidopsis.R37GM060398 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI JACOBSEN, STEVEN E · 2009 to 2018
$3.0M
Genetics of DNA Methylation Patterning of ArabidopsisR01GM060398 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI JACOBSEN, STEVEN E · 2000 to 2008
$2.2M
DNA methylation and its relationship to gene expression and physiologic traitsR01GM095656 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI PELLEGRINI, MATTEO · 2011 to 2014
$1.8M
Howard Hughes Medical InstituteNICHD NIH HHS R01 HD058047NICHD NIH HHS R01HD058047NIGMS NIH HHS GM60398NIGMS NIH HHS R01 GM095656-01A1NIGMS NIH HHS R37 GM060398
6 · The paper itself

Abstract

Methylation of cytosines (5(me)C) is a widespread heritable DNA modification. During mammalian development, two global demethylation events are followed by waves of de novo DNA methylation. In vivo mechanisms of DNA methylation establishment are largely uncharacterized. Here, we use Saccharomyces cerevisiae as a system lacking DNA methylation to define the chromatin features influencing the activity of the murine DNMT3B. Our data demonstrate that DNMT3B and H3K4 methylation are mutually exclusive and that DNMT3B is co-localized with H3K36 methylated regions. In support of this observation, DNA methylation analysis in yeast strains without Set1 and Set2 shows an increase of relative 5(me)C levels at the transcription start site and a decrease in the gene-body, respectively. We extend our observation to the murine male germline, where H3K4me3 is strongly anti-correlated while H3K36me3 correlates with accelerated DNA methylation. These results show the importance of H3K36 methylation for gene-body DNA methylation in vivo.

Indexed as

DNA MethylationAnimalsChromatinCpG IslandsCytosineDNA (Cytosine-5-)-MethyltransferasesDNA Methyltransferase 3BEmbryo, MammalianGene Expression Regulation, DevelopmentalGenetic VectorsGerm CellsHistone-Lysine N-MethyltransferaseHistonesMaleMethyltransferasesMiceChromatinCytosineDNA (Cytosine-5-)-MethyltransferasesDNA Methyltransferase 3BHistone-Lysine N-MethyltransferaseHistonesMethyltransferasesProtein IsoformsSaccharomyces cerevisiae ProteinsSET1 protein, S cerevisiaeSet2 protein, S cerevisiaedevelopmental biologyDNA methylationDNMT3evolutionary biologygenomicsH3K36me3H3K4me3histonemouseS. cerevisiaestem cells

Identifiers

PMID25848745
PMCPMC4412109
OpenAlexW2087785865

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.