ArticleIranian journal of microbiology2014
Development and evaluation of a Quadruplex Taq Man real-time PCR assay for simultaneous detection of clinical isolates of Enterococcus faecalis, Enterococcus faecium and their vanA and vanB genotypes.
Article in Iranian journal of microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Prevalence of vancomycin-resistant Enterococcus in Iran: a systematic review and meta-analysis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016Pooled it
- Implementation of wastewater-based epidemiology in a Northern California acute care hospital: a pilot study, June-July 2024.The Journal of hospital infection · 2026Article
- The potential of genome-oriented blood culture surveillance of vancomycin-resistant enterococcus faecium (VRE) to mirror local VRE epidemiology: a retrospective analysis and systematic comparison of VRE blood culture and VRE first patient isolates.BMC infectious diseases · 2025Article
- Spread of the novel vancomycin-resistantEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2025Observational
- Vancomycin-resistant Enterococcus infections in a hospital in Salvador, Bahia: a descriptive study, 2021-2023.Epidemiologia e servicos de saude : revista do Sistema Unico de Saude do Brasil · 2025Article
- Evaluation of PhenoMATRIX® PLUS for the culture-based rectal screening of CPOs, ESBL-E,Frontiers in microbiology · 2025Article
- Antimicrobial resistance patterns, virulence genes, and biofilm formation in enterococci strains collected from different sources.BMC infectious diseases · 2024Article
- Introduction and spread of vancomycin-resistant Enterococcus faecium (VREfm) at a German tertiary care medical center from 2004 until 2010: a retrospective whole-genome sequencing (WGS) study of the molecular epidemiology of VREfm.Antimicrobial resistance and infection control · 2024Article
- Multi-epitope chimeric vaccine designing and novel drug targets prioritization against multi-drug resistantFrontiers in microbiology · 2022Article
- An inter-laboratory study to investigate the impact of the bioinformatics component on microbiome analysis using mock communities.Scientific reports · 2021Article
- Assessment of Susceptibility to Five Common Antibiotics and Their Resistance Pattern in Clinical Enterococcus Isolates.Iranian journal of pathology · 2020Article
- High incidence of virulence determinants, aminoglycoside and vancomycin resistance in enterococci isolated from hospitalized patients in Northwest Iran.BMC infectious diseases · 2019Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
objectivesWe developed and evaluated the utility of a quadruplex Taqman real-time PCR assay that allows simultaneous identification of vancomycin-resistant genotypes and clinically relevant enterococci. MATERIALS AND
methodsThe specificity of the assay was tested using reference strains of vancomycin-resistant and susceptible enterococci. In total, 193 clinical isolates were identified and subsequently genotyped using a Quadruplex Taqman real-time PCR assay and melting curve analysis. Representative Quadruplex Taqman real-time PCR amplification curve were obtained for Enterococcus faecium, Enterococcus faecalis, vanA-containing E. faecium, vanB-containing E. faecalis.
resultsPhenotypic and genotypic analysis of the isolates gave same results for 82 enterococcal isolates, while in 5 isolates, they were inconsistent. We had three mixed strains, which were detected by the TaqMan real-time PCR assay and could not be identified correctly using phenotypic methods.
conclusionVancomycin resistant enterococci (VRE) genotyping and identification of clinically relevant enterococci were rapidly and correctly performed using TaqMan real-time multiplex real-time PCR assay.
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25848524PMC4385574What OpenQuestion holds
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