Evaluation of FLASH - PCR forrapid detection of Mycobacterium tuberculosis from clinical specimens.

Abbas Ali Imani Fooladi, Yousef Tarvedi Zadeh, Rezvan Mehrab, Raheleh Halabian, Taghi Azizi

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Article in Iranian journal of microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 authors.

Abbas Ali Imani FooladiApplied Microbiology, Research Center Baqiyatallah University of Medical Sciences, Tehran, Iran.

Yousef Tarvedi ZadehDepartment of Biology, Faculty of Basic Sciences, Imam Hussein University, Tehran, Iran.

Rezvan MehrabApplied Microbiology, Research Center Baqiyatallah University of Medical Sciences, Tehran, Iran.

Raheleh HalabianApplied Microbiology, Research Center Baqiyatallah University of Medical Sciences, Tehran, Iran.

Taghi AziziDepartment of Pathology, Schoo of Medical Sciences, Baqiyatallah University of Medical Sciences,Tehran, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND AND

objectivesTuberculosis (TB) is the oldest known bacterial disease in humans. Due to the rise of morbidity in recent years, early diagnosis of the disease is necessary. MATERIALS AND

methodsIn this study we used Fluorescent Amplification-Based Specific Hybridization (FLASH) PCR to targetIS6110 for rapid detection of M. tuberculosis (MTB). To investigate the important factors influencing the risk of TB, data from patients and their medical records were analyzed.

resultThe sensitivity and specificity of FLASH-PCR for detecting MTB were determined as 93.33% and 92.5%, respectively. The findings of this study have suggested that removal of the contaminants in FLASH-PCR sign ificantly reduced the detection time, and MTB was much more rapidly detected in the clinical specimens compared to the conventional culture and smear examination. Results of the medical survey showed that the majority of TB patients were males, over 51 years old, smokers, with pulmonary TB and normal chest X-ray (CXR).

conclusionMTB can be rapidly detected inclinical specimens using FLASH-PCR in comparison with culture and smear examination.

Indexed as

FLASH-PCRMycobacterium tuberculosisRapid detectionTuberculosis

Identifiers

PMID25848509

PMCPMC4385165

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.