ArticleIranian journal of microbiology2013
Molecular diversity of Mycobacterium tuberculosis strains indifferent provinces of Iran.
Article in Iranian journal of microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed.
- Preliminary study of molecular identification of Mycobacterium bovis from cow's milk in Lorestan (Iran).Scientific reports · 2024Article
- Mycobacterium tuberculosis genotypes in an ethnically diverse area with millions of pilgrims and thousands of immigrants.BMC infectious diseases · 2023Article
- A pilot study on the genetic diversity ofJournal of clinical tuberculosis and other mycobacterial diseases · 2020Article
- Role of Immigration in Tuberculosis Transmission to Iran: A Systematic Review.International journal of preventive medicine · 2020Review
- Article
- Challenge in direct Spoligotyping of Mycobacterium tuberculosis: a problematic issue in the region with high prevalence of polyclonal infections.BMC research notes · 2018Article
- Control of zoonotic cutaneous leishmaniasis vector, Phlebotomus papatasi, using attractive toxic sugar baits (ATSB).PloS one · 2017Article
- Molecular typing methods used in studies ofIranian journal of microbiology · 2016Review
- Drug Resistance Pattern of Mycobacterium tuberculosis Isolates From Patients Referred to TB Reference Laboratory in Ahvaz.Osong public health and research perspectives · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
objectivesMolecular epidemiology tools are widely used in determining epidemiology of tuberculosis. Spoligotyping is a molecular epidemiology method that is used for characterization and typing of Mycobacterium tuberculosis complex strains. The method is based on polymorphism of the chromosomal DR locus consisting of identical 36-bp DRs alternating with 35-41 unique spacers. The objective of this study was to investigate the prevalence of M. tuberculosis spoligotypes in different provinces of Iran. MATERIALS AND
methodsM. tuberculosis strains were isolated from TB patients of Mycobacteriology Research center (MRC). DNA was extracted from patient's clinical samples. PCR was performed by using of specific primers for DR region. The amplified DNA was hybridized to the spoligotyping Membrane. Hybridized DNA was detected with ECL detection kit and by exposing ECL Hyperfilm to the membrane. The obtained result was entered to a binary format and was analyzed using SpolDB4 database.
resultsSpoligotyping resulted in 136 different patterns. Out of 1242 M. tuberculosis strains, 1165 strains (93.8%) were classified into 59 clusters and the remaining strains (6.2 %) were singleton.
conclusionsThe results of present study showed that strains of CAS family were more prevalent than other strains in Iran. Other prevalent families were Haarlem, T and Beijing, respectively.
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Identifiers
25848506PMC4385162What OpenQuestion holds
Registered trials
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