Evidence map›Paper›PMID 25848385›Full record

ArticleIranian journal of parasitology

Acanthamoeba species in Swimming Pools of Cairo, Egypt.

Ahmad Al-Herrawy, Mahmoud Bahgat, Abd-Elhafez Mohammed, Ameen Ashour, Wafaa Hikal

Open access · greenAbstract read
In one paragraph

Article in Iranian journal of parasitology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed, 1 pooled it
2.9field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Article
  4. Article
  5. Isolation, identification of pathogenicJournal of advanced veterinary and animal research · 2018
    Article
  6. Article
  7. Article
  8. Water related ocular diseases.Saudi journal of ophthalmology : official journal of the Saudi Ophthalmological Society
    Review
  9. Article
  10. Surveillance and Molecular Identification ofIranian journal of parasitology
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Ahmad Al-HerrawyParasitology Laboratory, Water Pollution Research Department, NRC, 12622 Dokki, Giza, Egypt.
Mahmoud BahgatTherapeutic Chemistry Department, NRC, 12622 Dokki, Giza, Egypt.
Abd-Elhafez MohammedZoology Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
Ameen AshourZoology Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
Wafaa HikalParasitology Laboratory, Water Pollution Research Department, NRC, 12622 Dokki, Giza, Egypt.
Ain Shams University · EGNational Research Centre · EG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe free-living amoebae Acanthamoeba spp. have been recognized as etiologic agents of amoebic encephalitis, keratitis, otitis, lung lesions and other skin infections mainly in immuno-compromised individuals. The purpose of this study is to detect the presence of Acanthamoeba in swimming pools in Egypt using a polymerase chain reaction (PCR) method.

methodsWater samples were collected from 10 different swimming pools in Cairo, Egypt. Samples were cultured on non-nutrient agar for the detection of Acanthamoeba isolates that were confirmed by PCR amplification using genus specific primers. The molecularly confirmed Acanthamoeba isolates were morphologically identified to the species level.

resultsMembers of genus Acanthamoeba were detected in 49.2% of the examined swimming-pool water samples. Morphologically, six Acanthamoeba species were isolated from the examined swimming pool water namely A. polyphaga, A.castellanii, A. rhysodes, A. mauritaniensis, A. royreba and A. triangularis. All the identified species of Acanthamoeba were molecularly confirmed to be related to the genus Acanthamoeba.

conclusionThe isolated species of Acanthamoeba could provoke variable degrees of infections to the swimmers. The culture method is cheaper and easier than PCR techniques that are faster for the detection of free-living amoebae.

Indexed as

AcanthamoebaCyst morphologyEgyptPCRSwimming pools

Identifiers

PMID25848385
PMCPMC4386039
OpenAlexW2132308072

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.