ArticleMolecular and cellular biology2015
Role for Protein Kinase A in the Neurospora Circadian Clock by Regulating White Collar-Independent frequency Transcription through Phosphorylation of RCM-1.
Article in Molecular and cellular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 31 citations in OpenAlex.
- Heterotrimeric G-proteins and cAMP regulate gene expression during growth on cellulose inmBio · 2026Article
- Paf1C regulates the Neurospora circadian clock by promoting the transcription elongation of frequency.PLoS genetics · 2025Article
- Phase Separation-Regulated Fungal Growth, Sexual Development, Adaptation and Synthetic Biology Applications.Journal of fungi (Basel, Switzerland) · 2025Review
- Functional Study of cAMP-Dependent Protein Kinase A inJournal of fungi (Basel, Switzerland) · 2023Article
- Methylxanthines Modulate Circadian Period Length Independently of the Action of Phosphodiesterase.Microbiology spectrum · 2023Article
- The Frq-Frh Complex Light-Dependently Delays Sfl1-Induced Microsclerotia Formation inJournal of fungi (Basel, Switzerland) · 2023Article
- Current genetic strategies to investigate gene functions in Trichoderma reesei.Microbial cell factories · 2023Review
- Nutritional compensation of the circadian clock is a conserved process influenced by gene expression regulation and mRNA stability.PLoS biology · 2023Article
- Integrative Role ofInternational journal of molecular sciences · 2021Article
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- Interdependent recruitment of CYC8/TUP1 and the transcriptional activator XYR1 at target promoters is required for induced cellulase gene expression in Trichoderma reesei.PLoS genetics · 2021Article
- ECHO: an application for detection and analysis of oscillators identifies metabolic regulation on genome-wide circadian output.Bioinformatics (Oxford, England) · 2020Article
- FRQ-CK1 interaction determines the period of circadian rhythms in Neurospora.Nature communications · 2019Article
- Article
- The role of PKAc1 in gene regulation and trichodimerol production inFungal biology and biotechnology · 2019Article
- Non-transcriptional processes in circadian rhythm generation.Current opinion in physiology · 2018Review
- Transcription factor CBF-1 is critical for circadian gene expression by modulating WHITE COLLAR complex recruitment to the frq locus.PLoS genetics · 2018Article
- Spatio-temporal MAPK dynamics mediate cell behavior coordination during fungal somatic cell fusion.Journal of cell science · 2018Article
- DNA Replication Is Required for Circadian Clock Function by Regulating Rhythmic Nucleosome Composition.Molecular cell · 2017Article
- Transcriptional repression of frequency by the IEC-1-INO80 complex is required for normal Neurospora circadian clock function.PLoS genetics · 2017Article
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10 authors at 5 institutions in 2 countries.
Funding
Abstract
Rhythmic activation and repression of clock gene expression is essential for the eukaryotic circadian clock functions. In the Neurospora circadian oscillator, the transcription of the frequency (frq) gene is periodically activated by the White Collar (WC) complex and suppressed by the FRQ-FRH complex. We previously showed that there is WC-independent frq transcription and its repression is required for circadian gene expression. How WC-independent frq transcription is regulated is not known. We show here that elevated protein kinase A (PKA) activity results in WC-independent frq transcription and the loss of clock function. We identified RCM-1 as the protein partner of RCO-1 and an essential component of the clock through its role in suppressing WC-independent frq transcription. RCM-1 is a phosphoprotein and is a substrate of PKA in vivo and in vitro. Mutation of the PKA-dependent phosphorylation sites on RCM-1 results in WC-independent transcription of frq and impaired clock function. Furthermore, we showed that RCM-1 is associated with the chromatin at the frq locus, a process that is inhibited by PKA. Together, our results demonstrate that PKA regulates frq transcription by inhibiting RCM-1 activity through RCM-1 phosphorylation.
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