ArticleProceedings of the National Academy of Sciences of the United States of America2015
High-yield hydrogen production from biomass by in vitro metabolic engineering: Mixed sugars coutilization and kinetic modeling.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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40 citing papers in PubMed, 228 citations in OpenAlex.
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- Quantitative Online Monitoring of an Immobilized Enzymatic Network by Ion Mobility-Mass Spectrometry.Journal of the American Chemical Society · 2024Article
- ATP-free in vitro biotransformation of starch-derived maltodextrin into poly-3-hydroxybutyrate via acetyl-CoA.Nature communications · 2024Article
- Conversion of acetate and glyoxylate to fumarate by a cell-free synthetic enzymatic biosystem.Synthetic and systems biotechnology · 2023Article
- Exploring the Feasibility of Cell-Free Synthesis as a Platform for Polyhydroxyalkanoate (PHA) Production: Opportunities and Challenges.Polymers · 2023Review
- Biomanufacturing by In Vitro Biotransformation (ivBT) Using Purified Cascade Multi-enzymes.Advances in biochemical engineering/biotechnology · 2023Article
- A lysate proteome engineering strategy for enhancing cell-free metabolite production.Metabolic engineering communications · 2021Article
- Microbial biotechnological approaches: renewable bioprocessing for the future energy systems.Microbial cell factories · 2021Review
- High-efficiency transformation of archaea by direct PCR products with its application to directed evolution of a thermostable enzyme.Microbial biotechnology · 2021Article
- An ATP-free in vitro synthetic enzymatic biosystem facilitating one-pot stoichiometric conversion of starch to mannitol.Applied microbiology and biotechnology · 2021Article
- Bioprospecting of microbial strains for biofuel production: metabolic engineering, applications, and challenges.Biotechnology for biofuels · 2021Review
- Extremophilic Oxidoreductases for the Industry: Five Successful Examples With Promising Projections.Frontiers in bioengineering and biotechnology · 2021Review
- In vitro multi-enzymatic cascades using recombinant lysates of E. coli: an emerging biocatalysis platform.Biophysical reviews · 2020Review
- Macromolecular crowding effects on transcription and translation are regulated by free magnesium ion.Biotechnology and applied biochemistry · 2020Article
- A critical comparison of cellular and cell-free bioproduction systems.Current opinion in biotechnology · 2019Review
- Engineering a Seven Enzyme Biotransformation using Mathematical Modelling and Characterized Enzyme Parts.ChemCatChem · 2019Article
- Developing a single strain for in vitro salvage synthesis of NADMicrobial cell factories · 2019Article
- Biosynthesis of Raffinose and Stachyose from Sucrose via anApplied and environmental microbiology · 2019Article
- Metabolite-based mutualism enhances hydrogen production in a two-species microbial consortium.Communications biology · 2019Article
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of hydrogen (H2) as a fuel offers enhanced energy conversion efficiency and tremendous potential to decrease greenhouse gas emissions, but producing it in a distributed, carbon-neutral, low-cost manner requires new technologies. Herein we demonstrate the complete conversion of glucose and xylose from plant biomass to H2 and CO2 based on an in vitro synthetic enzymatic pathway. Glucose and xylose were simultaneously converted to H2 with a yield of two H2 per carbon, the maximum possible yield. Parameters of a nonlinear kinetic model were fitted with experimental data using a genetic algorithm, and a global sensitivity analysis was used to identify the enzymes that have the greatest impact on reaction rate and yield. After optimizing enzyme loadings using this model, volumetric H2 productivity was increased 3-fold to 32 mmol H2⋅L(-1)⋅h(-1). The productivity was further enhanced to 54 mmol H2⋅L(-1)⋅h(-1) by increasing reaction temperature, substrate, and enzyme concentrations--an increase of 67-fold compared with the initial studies using this method. The production of hydrogen from locally produced biomass is a promising means to achieve global green energy production.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.