ArticleInfection and immunity2015
Staphylococcus aureus induces hypoxia and cellular damage in porcine dermal explants.
Article in Infection and immunity, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 61 citations in OpenAlex.
- Reduced Oxygen Condition Is Associated with Genome-Wide Expression Changes in Mastitis-LineageInternational journal of molecular sciences · 2026Article
- Polysaccharides and Peptides With Wound Healing Activity From Bacteria and Fungi.Journal of basic microbiology · 2024Review
- Commensal microbiome promotes hair follicle regeneration by inducing keratinocyte HIF-1α signaling and glutamine metabolism.Science advances · 2023Article
- Activity of a hypochlorous acid-producing electrochemical bandage as assessed with a porcine explant biofilm model.Biotechnology and bioengineering · 2023Article
- NF-κB signaling pathway mechanism in cow intertoe skin inflammation caused byFrontiers in cellular and infection microbiology · 2023Article
- Anti-Biofilm Activity of a Tunable Hypochlorous Acid-Generating Electrochemical Bandage Controlled By a Wearable Potentiostat.Advanced engineering materials · 2023Article
- Antibiotics Limit Adaptation of Drug-Resistant Staphylococcus aureus to Hypoxia.Antimicrobial agents and chemotherapy · 2022Article
- Development of an implantable three-dimensional model of a functional pathogenic multispecies biofilm to study infected wounds.Scientific reports · 2022Article
- An Altered Skin and Gut Microbiota Are Involved in the Modulation of Itch in Atopic Dermatitis.Cells · 2022Review
- Efficacy and toxicity of hydrogen peroxide producing electrochemical bandages in a porcine explant biofilm model.Journal of applied microbiology · 2022Article
- Immunometabolism in biofilm infection: lessons from cancer.Molecular medicine (Cambridge, Mass.) · 2022Review
- The Influence of Microbiome Dysbiosis and Bacterial Biofilms on Epidermal Barrier Function in Atopic Dermatitis-An Update.International journal of molecular sciences · 2021Review
- Strong Antimicrobial and Healing Effects of Beta-Acids from Hops in Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2021Article
- Lower Airway Dysbiosis Affects Lung Cancer Progression.Cancer discovery · 2021Article
- Studying Neutrophil Function in vitro: Cell Models and Environmental Factors.Journal of inflammation research · 2021Review
- Micrococcal Nuclease stimulatesFrontiers in cellular and infection microbiology · 2021Article
- Encapsulation of collagen mimetic peptide-tethered vancomycin liposomes in collagen-based scaffolds for infection control in wounds.Acta biomaterialia · 2020Article
- Direct Microscopic Observation of Human Neutrophil-Staphylococcus aureus InteractionInfection and immunity · 2019Article
- The Impact of Hypoxia on the Host-Pathogen Interaction between Neutrophils andInternational journal of molecular sciences · 2019Review
- Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
We developed a porcine dermal explant model to determine the extent to which Staphylococcus aureus biofilm communities deplete oxygen, change pH, and produce damage in underlying tissue. Microelectrode measurements demonstrated that dissolved oxygen (DO) in biofilm-free dermal tissue was 4.45 ± 1.17 mg/liter, while DO levels for biofilm-infected tissue declined sharply from the surface, with no measurable oxygen detectable in the underlying dermal tissue. Magnetic resonance imaging demonstrated that biofilm-free dermal tissue had a significantly lower relative effective diffusion coefficient (0.26 ± 0.09 to 0.30 ± 0.12) than biofilm-infected dermal tissue (0.40 ± 0.12 to 0.48 ± 0.12; P < 0.0001). Thus, the difference in DO level was attributable to biofilm-induced oxygen demand rather than changes in oxygen diffusivity. Microelectrode measures showed that pH within biofilm-infected explants was more alkaline than in biofilm-free explants (8.0 ± 0.17 versus 7.5 ± 0.15, respectively; P < 0.002). Cellular and nuclear details were lost in the infected explants, consistent with cell death. Quantitative label-free shotgun proteomics demonstrated that both proapoptotic programmed cell death protein 5 and antiapoptotic macrophage migration inhibitory factor accumulated in the infected-explant spent medium, compared with uninfected-explant spent media (1,351-fold and 58-fold, respectively), consistent with the cooccurrence of apoptosis and necrosis in the explants. Biofilm-origin proteins reflected an extracellular matrix-adapted lifestyle of S. aureus. S. aureus biofilms deplete oxygen, increase pH, and induce cell death, all factors that contribute to impede wound healing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.