ArticleThe Journal of biological chemistry2015
Two duplicated genes DDI2 and DDI3 in budding yeast encode a cyanamide hydratase and are induced by cyanamide.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Zinc-finger transcription factor Fzf1 binds to its target DNA in a non-canonical manner.Nucleic acids research · 2026Article
- In-Cell Proteomics Enables High-Resolution Temporal Profiling of Cell Cycle Progression and DNA Damage Response in Saccharomyces cerevisiae.Proteomics · 2026Article
- Zinc-Finger 5 Is an Activation Domain in theJournal of fungi (Basel, Switzerland) · 2025Article
- Article
- Uniform [Journal of biomolecular NMR · 2024Article
- Transcriptional activation of budding yeast DDI2/3 through chemical modifications of Fzf1.Cell biology and toxicology · 2023Article
- Comparative Genomics Supports That Brazilian BioethanolFrontiers in microbiology · 2021Article
- Draft genome of Meyerozyma guilliermondii strain vka1: a yeast strain with composting potential.Journal, genetic engineering & biotechnology · 2020Article
- Fine-tuning the expression of target genes using a DDI2 promoter gene switch in budding yeast.Scientific reports · 2019Article
- Structure of Ddi2, a highly inducible detoxifying metalloenzyme fromThe Journal of biological chemistry · 2019Article
- Utilization of a Strongly InducibleFrontiers in microbiology · 2018Article
- Comparative genomics of Beauveria bassiana: uncovering signatures of virulence against mosquitoes.BMC genomics · 2016Article
- Genome Sequence and Analysis of a Stress-Tolerant, Wild-Derived Strain of Saccharomyces cerevisiae Used in Biofuels Research.G3 (Bethesda, Md.) · 2016Article
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6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two DNA damage-inducible genes in Saccharomyces cerevisiae, DDI2 and DDI3, are identical and encode putative HD domain-containing proteins, whose functions are currently unknown. Because Ddi2/3 also shows limited homology to a fungal cyanamide hydratase that converts cyanamide to urea, we tested the enzymatic activity of recombinant Ddi2. To this end, we developed a novel enzymatic assay and determined that the Km value of the recombinant Ddi2/3 for cyanamide is 17.3 ± 0.05 mm, and its activity requires conserved residues in the HD domain. Unlike most other DNA damage-inducible genes, DDI2/3 is only induced by a specific set of alkylating agents and surprisingly is strongly induced by cyanamide. To characterize the biological function of DDI2/3, we sequentially deleted both DDI genes and found that the double mutant was unable to metabolize cyanamide and became much more sensitive to growth inhibition by cyanamide, suggesting that the DDI2/3 genes protect host cells from cyanamide toxicity. Despite the physiological relevance of the cyanamide induction, DDI2/3 is not involved in its own transcriptional regulation. The significance of cyanamide hydratase activity and its induced expression is discussed.
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