ArticleDevelopmental neurobiology2015
Nerve growth factor promotes reorganization of the axonal microtubule array at sites of axon collateral branching.
Article in Developmental neurobiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 46 citations in OpenAlex.
- Nerve growth factor signaling tunes axon maintenance protein abundance and kinetics of Wallerian degeneration.Molecular biology of the cell · 2025Article
- Nerve Growth Factor Signaling Tunes Axon Maintenance Protein Abundance and Kinetics of Wallerian Degeneration.bioRxiv : the preprint server for biology · 2025Article
- Vascular smooth muscle cell-derived nerve growth factor regulates sympathetic collateral branching to innervate blood vessels in embryonic skin.Biology open · 2024Article
- Article
- Local mRNA translation and cytoskeletal reorganization: Mechanisms that tune neuronal responses.Frontiers in molecular neuroscience · 2022Review
- Astragaloside‑IV modulates NGF‑induced osteoblast differentiation via the GSK3β/β‑catenin signalling pathway.Molecular medicine reports · 2021Article
- The GTPase Arl8B Plays a Principle Role in the Positioning of Interstitial Axon Branches by Spatially Controlling Autophagosome and Lysosome Location.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Article
- [A differential peptidomics analysis of hippocampal tissue in a rat model of premature white matter injury].Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics · 2019Article
- MAP7 Prevents Axonal Branch Retraction by Creating a Stable Microtubule Boundary to Rescue Polymerization.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Understanding the axonal response to injury by in vivo imaging in the mouse spinal cord: A tale of two branches.Experimental neurology · 2019Review
- Distal Axonal Proteins and Their Related MiRNAs in Cultured Cortical Neurons.Molecular neurobiology · 2019Article
- ALS-Linked SOD1 Mutants Enhance Neurite Outgrowth and Branching in Adult Motor Neurons.iScience · 2019Article
- Article
- It takes a village to raise a branch: Cellular mechanisms of the initiation of axon collateral branches.Molecular and cellular neurosciences · 2017Review
- CSPGs inhibit axon branching by impairing mitochondria-dependent regulation of actin dynamics and axonal translation.Developmental neurobiology · 2017Article
- MAP7 Regulates Axon Collateral Branch Development in Dorsal Root Ganglion Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- The Kinesin Adaptor Calsyntenin-1 Organizes Microtubule Polarity and Regulates Dynamics during Sensory Axon Arbor Development.Frontiers in cellular neuroscience · 2017Article
- Beyond the cytoskeleton: The emerging role of organelles and membrane remodeling in the regulation of axon collateral branches.Developmental neurobiology · 2016Review
- Drebrin coordinates the actin and microtubule cytoskeleton during the initiation of axon collateral branches.Developmental neurobiology · 2016Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The localized debundling of the axonal microtubule array and the entry of microtubules into axonal filopodia are two defining features of collateral branching. We report that nerve growth factor (NGF), a branch-inducing signal, increases the frequency of microtubule debundling along the axon shaft of chicken embryonic sensory neurons. Sites of debundling correlate strongly with the localized targeting of microtubules into filopodia. Platinum replica electron microscopy suggests physical interactions between debundled microtubules and axonal actin filaments. However, as evidenced by depolymerization of actin filaments and inhibition of myosin II, actomyosin force generation does not promote debundling. In contrast, loss of actin filaments or inhibition of myosin II activity promotes debundling, indicating that axonal actomyosin forces suppress debundling. MAP1B is a microtubule associated protein that represses axon branching. Following treatment with NGF, microtubules penetrating filopodia during the early stages of branching exhibited lower levels of associated MAP1B. NGF increased and decreased the levels of MAP1B phosphorylated at a GSK-3β site (pMAP1B) along the axon shaft and within axonal filopodia, respectively. The levels of MAP1B and pMAP1B were not altered at sites of debundling, relative to the rest of the axon. Unlike the previously determined effects of NGF on the axonal actin cytoskeleton, the effects of NGF on microtubule debundling were not affected by inhibition of protein synthesis. Collectively, these data indicate that NGF promotes localized axonal microtubule debundling, that actomyosin forces antagonize microtubule debundling, and that NGF regulates pMAP1B in axonal filopodia during the early stages of collateral branch formation.
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