ArticlePlant biotechnology journal2016
Product stability and sequestration mechanisms in Solanum tuberosum engineered to biosynthesize high value ketocarotenoids.
Article in Plant biotechnology journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Biofortification of potato nutrition.Journal of advanced research · 2025Review
- Metabolically engineered plant cell cultures as biofactories for the production of high-value carotenoids astaxanthin and canthaxanthin.Scientific reports · 2025Article
- Effects of high light exposure and heterologous expression of β-carotene ketolase on the metabolism of carotenoids inFrontiers in bioengineering and biotechnology · 2025Article
- Cell-penetrating peptides for sustainable agriculture.Trends in plant science · 2024Review
- Ketocarotenoid production in tomato triggers metabolic reprogramming and cellular adaptation: The quest for homeostasis.Plant biotechnology journal · 2024Article
- Carotenoid composition and sequestration in cassava (Manihot esculentum Crantz) roots.PloS one · 2024Article
- Integrative analysis of the pharmaceutical active ingredient and transcriptome of the aerial parts of Glycyrrhiza uralensis under salt stress reveals liquiritin accumulation via ABA-mediated signaling.Molecular genetics and genomics : MGG · 2022Article
- Plant carotenoids: recent advances and future perspectives.Molecular horticulture · 2022Review
- Riboswitch-mediated inducible expression of an astaxanthin biosynthetic operon in plastids.Plant physiology · 2022Article
- Nucleotide variation in the phytoene synthase (ClPsy1) gene contributes to golden flesh in watermelon (Citrullus lanatus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Construction of transgenicPlant biotechnology (Tokyo, Japan) · 2021Article
- Engineering a Plant-Derived Astaxanthin Synthetic Pathway IntoFrontiers in plant science · 2021Article
- Canthaxanthin, a Red-Hot Carotenoid: Applications, Synthesis, and Biosynthetic Evolution.Plants (Basel, Switzerland) · 2020Review
- Quantitative trait loci and differential gene expression analyses reveal the genetic basis for negatively associated β-carotene and starch content in hexaploid sweetpotato [Ipomoea batatas (L.) Lam.].TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- The road to astaxanthin production in tomato fruit reveals plastid and metabolic adaptation resulting in an unintended high lycopene genotype with delayed over-ripening properties.Plant biotechnology journal · 2019Article
- Construction of a fusion enzyme for astaxanthin formation and its characterisation in microbial and plant hosts: A new tool for engineering ketocarotenoids.Metabolic engineering · 2019Article
- Engineering of tomato for the sustainable production of ketocarotenoids and its evaluation in aquaculture feed.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To produce commercially valuable ketocarotenoids in Solanum tuberosum, the 4, 4' β-oxygenase (crtW) and 3, 3' β-hydroxylase (crtZ) genes from Brevundimonas spp. have been expressed in the plant host under constitutive transcriptional control. The CRTW and CRTZ enzymes are capable of modifying endogenous plant carotenoids to form a range of hydroxylated and ketolated derivatives. The host (cv. Désirée) produced significant levels of nonendogenous carotenoid products in all tissues, but at the apparent expense of the economically critical metabolite, starch. Carotenoid levels increased in both wild-type and transgenic tubers following cold storage; however, stability during heat processing varied between compounds. Subcellular fractionation of leaf tissues revealed the presence of ketocarotenoids in thylakoid membranes, but not predominantly in the photosynthetic complexes. A dramatic increase in the carotenoid content of plastoglobuli was determined. These findings were corroborated by microscopic analysis of chloroplasts. In tuber tissues, esterified carotenoids, representing 13% of the total pigment found in wild-type extracts, were sequestered in plastoglobuli. In the transgenic tubers, this proportion increased to 45%, with esterified nonendogenous carotenoids in place of endogenous compounds. Conversely, nonesterified carotenoids in both wild-type and transgenic tuber tissues were associated with amyloplast membranes and starch granules.
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