ArticleNucleic acids research2015
Nuclear pore components affect distinct stages of intron-containing gene expression.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- DNA lesions can frequently precede DNA:RNA hybrid accumulation.Nature communications · 2025Article
- Single-molecule quantitation of RNA-binding protein occupancy and stoichiometry defines a role for Yra1 (Aly/REF) in nuclear mRNP organization.Cell reports · 2023Article
- An evolutionarily conserved bimodular domain anchors ZC3HC1 and its yeast homologue Pml39p to the nuclear basket.Molecular biology of the cell · 2023Article
- PABPN1 prevents the nuclear export of an unspliced RNA with a constitutive transport element and controls human gene expression via intron retention.RNA (New York, N.Y.) · 2023Article
- Structure of the pre-mRNA leakage 39-kDa protein reveals a single domain of integrated zf-C3HC and Rsm1 modules.Scientific reports · 2022Article
- Nuclear mRNA metabolism drives selective basket assembly on a subset of nuclear pore complexes in budding yeast.Molecular cell · 2022Article
- DNA circles promote yeast ageing in part through stimulating the reorganization of nuclear pore complexes.eLife · 2022Article
- Analysis of Ubiquitylation and SUMOylation of Yeast Nuclear Pore Complex Proteins.Methods in molecular biology (Clifton, N.J.) · 2022Article
- A nuclear pore sub-complex restricts the propagation of Ty retrotransposons by limiting their transcription.PLoS genetics · 2021Article
- Article
- High-Resolution Microscopy to Learn the Nuclear Organization of the Living Yeast Cells.Stem cells international · 2021Article
- Recruitment of an Activated Gene to the Yeast Nuclear Pore Complex Requires Sumoylation.Frontiers in genetics · 2020Article
- Polyadenylation and nuclear export of mRNAs.The Journal of biological chemistry · 2019Review
- Structure-function relationships in the Nab2 polyadenosine-RNA binding Zn finger protein family.Protein science : a publication of the Protein Society · 2019Review
- The mRNA export adaptor Yra1 contributes to DNA double-strand break repair through its C-box domain.PloS one · 2019Article
- A Rev-CBP80-eIF4AI complex drives Gag synthesis from the HIV-1 unspliced mRNA.Nucleic acids research · 2018Article
- A SUMO-dependent feedback loop senses and controls the biogenesis of nuclear pore subunits.Nature communications · 2018Article
- Quality control of mRNAs at the entry of the nuclear pore: Cooperation in a complex molecular system.Nucleus (Austin, Tex.) · 2018Review
- An NXF1 mRNA with a retained intron is expressed in hippocampal and neocortical neurons and is translated into a protein that functions as an Nxf1 cofactor.Molecular biology of the cell · 2016Article
- Intron retention-dependent gene regulation in Cryptococcus neoformans.Scientific reports · 2016Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several nuclear pore-associated factors, including the SUMO-protease Ulp1, have been proposed to prevent the export of intron-containing messenger ribonucleoparticles (mRNPs) in yeast. However, the molecular mechanisms of this nuclear pore-dependent mRNA quality control, including the sumoylated targets of Ulp1, have remained unidentified. Here, we demonstrate that the apparent 'pre-mRNA leakage' phenotype arising upon ULP1 inactivation is shared by sumoylation mutants of the THO complex, an early mRNP biogenesis factor. Importantly, we establish that alteration of THO complex activity differentially impairs the expression of intronless and intron-containing reporter genes, rather than triggering bona fide 'pre-mRNA leakage'. Indeed, we show that the presence of introns within THO target genes attenuates the effect of THO inactivation on their transcription. Epistasis analyses further clarify that different nuclear pore components influence intron-containing gene expression at distinct stages. Ulp1, whose maintenance at nuclear pores depends on the Nup84 complex, impacts on THO-dependent gene expression, whereas the nuclear basket-associated Mlp1/Pml39 proteins prevent pre-mRNA export at a later stage, contributing to mRNA quality control. Our study thus highlights the multiplicity of mechanisms by which nuclear pores contribute to gene expression, and further provides the first evidence that intronic sequences can alleviate early mRNP biogenesis defects.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.