ArticleNucleic acids research2015
A universal strategy for regulating mRNA translation in prokaryotic and eukaryotic cells.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 34 citations in OpenAlex.
- Remote Control of Eukaryotic Gene Expression by a Modular Ultrasound-Responsive RNA Toolkit.Angewandte Chemie (International ed. in English) · 2025Article
- Probing the orthogonality and robustness of the mammalian RNA-binding protein Musashi-1 in Escherichia coli.Journal of biological engineering · 2024Article
- Repurposing the mammalian RNA-binding protein Musashi-1 as an allosteric translation repressor in bacteria.eLife · 2024Article
- Simultaneous multifunctional transcriptome engineering by CRISPR RNA scaffold.Nucleic acids research · 2023Article
- The potential of engineered eukaryotic RNA-binding proteins as molecular tools and therapeutics.Wiley interdisciplinary reviews. RNA · 2020Review
- Modulation of miRNA function by natural and synthetic RNA-binding proteins in cancer.Cellular and molecular life sciences : CMLS · 2019Review
- A Yeast System for Discovering Optogenetic Inhibitors of Eukaryotic Translation Initiation.ACS synthetic biology · 2019Article
- Synthetic mRNA devices that detect endogenous proteins and distinguish mammalian cells.Nucleic acids research · 2017Article
- RNA-based gene circuits for cell regulation.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2016Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
We describe a simple strategy to control mRNA translation in both prokaryotic and eukaryotic cells which relies on a unique protein-RNA interaction. Specifically, we used the Pumilio/FBF (PUF) protein to repress translation by binding in between the ribosome binding site (RBS) and the start codon (in Escherichia coli), or by binding to the 5' untranslated region of target mRNAs (in mammalian cells). The design principle is straightforward, the extent of translational repression can be tuned and the regulator is genetically encoded, enabling the construction of artificial signal cascades. We demonstrate that this approach can also be used to regulate polycistronic mRNAs; such regulation has rarely been achieved in previous reports. Since the regulator used in this study is a modular RNA-binding protein, which can be engineered to target different 8-nucleotide RNA sequences, our strategy could be used in the future to target endogenous mRNAs for regulating metabolic flows and signaling pathways in both prokaryotic and eukaryotic cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.