Evidence map›Paper›PMID 25845539›Full record

ArticleJournal of microbiology (Seoul, Korea)2015

Effect of promoter-upstream sequence on σ38-dependent stationary phase gene transcription.

Hyung-Ju Lim, Kwangsoo Kim, Minsang Shin, Jae-Ho Jeong, Phil Youl Ryu, Hyon E Choy

Abstract read
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In one paragraph

Article in Journal of microbiology (Seoul, Korea), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Hyung-Ju LimDepartment of Microbiology, Chonnam National University Medical School, Kwangju, 501-746, Republic of Korea.
Kwangsoo Kim
Minsang Shin
Jae-Ho Jeong
Phil Youl Ryu
Hyon E Choy

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

σ38 in Escherichia coli is required for expression of a subset of stationary phase genes. However, the promoter elements for σ38-dependent genes are virtually indistinguishable from that for σ70-dependent house-keeping genes. hdeABp is a σ38-dependent promoter and LEE5p is a σ70-dependent promoter, but both are repressed by H-NS, a bacterial histone-like protein, which acts at promoter upstream sequence. We swapped the promoter upstream sequences of the two promoters and found that the σ dependency was switched. This was further verified using lacUV5 core promoter. The results suggested that the determinant for σ38-dependent promoter lies in the promoter upstream sequence.

Indexed as

Gene Expression Regulation, BacterialPromoter Regions, GeneticBacterial ProteinsBase SequenceEscherichia coliEscherichia coli ProteinsGenes, EssentialSigma FactorTranscription, GeneticBacterial ProteinsEscherichia coli ProteinsSigma Factorsigma factor KatF protein, Bacteria

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.