ArticleJournal of Zhejiang University. Science. B2015
Effect of culturing conditions on the expression of key enzymes in the proteolytic system of Lactobacillus bulgaricus.
Article in Journal of Zhejiang University. Science. B, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 20 citations in OpenAlex.
- Peptidomic Characterization of < 3 kDa Peptides from Milk Fermented by Lactic Acid Bacteria: Sequence-Based Antimicrobial Prediction and In Vitro Immuno-Oncological Evaluation.Probiotics and antimicrobial proteins · 2026Article
- Impact of lactic acid bacterial strain and storage temperature on the quality and functionality of fermented mulberry juice.Food chemistry: X · 2026Article
- A novel selective medium for isolation of Limosilactobacillus reuteri from dietary supplements.Journal of food and drug analysis · 2024Article
- Deciphering the metabolism ofApplied and environmental microbiology · 2024Article
- Molecular and biotechnological characteristics of proteolytic activity fromAIMS microbiology · 2023Article
- Complete Replacement of Nitrite With aFrontiers in microbiology · 2021Article
- Production of Bioactive Peptides byFrontiers in microbiology · 2018Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
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Abstract
The proteolytic system of Lactobacillus bulgaricus breaks down milk proteins into peptides and amino acids, which are essential for the growth of the bacteria. The aim of this study was to determine the expressions of seven key genes in the proteolytic system under different culturing conditions (different phases, initial pH values, temperatures, and nitrogen sources) using real-time polymerase chain reaction (RT-PCR). The transcriptions of the seven genes were reduced by 30-fold on average in the stationary phase compared with the exponential growth phase. The transcriptions of the seven genes were reduced by 62.5-, 15.0-, and 59.0-fold in the strains KLDS 08006, KLDS 08007, and KLDS 08012, respectively, indicating that the expressions of the seven genes were significantly different among strains. In addition, the expressions of the seven genes were repressed in the MRS medium containing casein peptone. The effect of peptone supply on PepX transcription was the weakest compared with the other six genes, and the impact on OppD transcription was the strongest. Moreover, the expressions of the seven genes were significantly different among different strains (P<0.05). All these results indicated that the culturing conditions affected the expression of the proteolytic system genes in Lactobacillus bulgaricus at the transcription level.
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