ArticleEnvironmental microbiology2015
Integrated whole-genome screening for Pseudomonas aeruginosa virulence genes using multiple disease models reveals that pathogenicity is host specific.
Article in Environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 54 citations in OpenAlex.
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- Characterization of Nematicidal Activity and Nematode-Toxic Metabolites of a SoilbornePathogens (Basel, Switzerland) · 2022Article
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- Identification of the Genes of the Plant PathogenFrontiers in microbiology · 2022Article
- Biofertilizer microorganisms accompanying pathogenic attributes: a potential threat.Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Review
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- Sanguinarine Inhibits the 2-Ketogluconate Pathway of Glucose Utilization inFrontiers in microbiology · 2021Article
- AhaP, A Quorum Quenching Acylase fromMarine drugs · 2021Article
- Identification of FDA-approved antivirulence drugs targeting theVirulence · 2020Article
- The evolution of virulence inProceedings. Biological sciences · 2020Article
- Shifts in microbial community, pathogenicity-related genes and antibiotic resistance genes during dairy manure piled up.Microbial biotechnology · 2020Article
- Multidrug Resistance (MDR) and Collateral Sensitivity in Bacteria, with Special Attention to Genetic and Evolutionary Aspects and to the Perspectives of Antimicrobial Peptides-A Review.Pathogens (Basel, Switzerland) · 2020Review
- Pyoverdine-Mediated Killing ofInternational journal of molecular sciences · 2020Article
- Antibiotic efficacy varies based on the infection model and treatment regimen forThe European respiratory journal · 2020Article
- Virulence Constitution of Multi-Drug-ResistantInfection and drug resistance · 2020Article
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Authors and funding
12 authors at 6 institutions in 4 countries.
Funding
Abstract
Pseudomonas aeruginosa is a multi-host opportunistic pathogen causing a wide range of diseases because of the armoury of virulence factors it produces, and it is difficult to eradicate because of its intrinsic resistance to antibiotics. Using an integrated whole-genome approach, we searched for P. aeruginosa virulence genes with multi-host relevance. We constructed a random library of 57 360 Tn5 mutants in P. aeruginosa PAO1-L and screened it in vitro for those showing pleiotropic effects in virulence phenotypes (reduced swarming, exo-protease and pyocyanin production). A set of these pleiotropic mutants were assayed for reduced toxicity in Drosophila melanogaster, Caenorhabditis elegans, human cell lines and mice. Surprisingly, the screening revealed that the virulence of the majority of P. aeruginosa mutants varied between disease models, suggesting that virulence is dependent on the disease model used and hence the host environment. Genomic analysis revealed that these virulence-related genes encoded proteins from almost all functional classes, which were conserved among P. aeruginosa strains. Thus, we provide strong evidence that although P. aeruginosa is capable of infecting a wide range of hosts, many of its virulence determinants are host specific. These findings have important implication when searching for novel anti-virulence targets to develop new treatments against P. aeruginosa.
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