ArticleOncotarget2015
Aquaporin 1 and 5 expression decreases during human intervertebral disc degeneration: Novel HIF-1-mediated regulation of aquaporins in NP cells.
Article in Oncotarget, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 27 citations in OpenAlex.
- Mapping the degenerating intervertebral disc: a systematic review of histological evidence.Frontiers in medicine · 2026Pooled it
- Short-Term Dynamic Unloading of Bovine Tail Discs in Culture Partially Mitigates Induced Degeneration After One-Strike Trigger.JOR spine · 2025Article
- Review
- Aquaporin-5 Dynamic Regulation.International journal of molecular sciences · 2023Review
- Aquaporins in Reproductive System.Advances in experimental medicine and biology · 2023Review
- Expression Regulation of Water Reabsorption Genes and Transcription Factors in the Kidneys ofFrontiers in physiology · 2022Article
- TonEBP regulates the hyperosmotic expression of aquaporin 1 and 5 in the intervertebral disc.Scientific reports · 2021Article
- Multiscale Regulation of the Intervertebral Disc: Achievements in Experimental, In Silico, and Regenerative Research.International journal of molecular sciences · 2021Review
- Immunohistochemical analysis of protein expression in formalin fixed paraffin embedded human intervertebral disc tissues.JOR spine · 2020Article
- Aquaporin expression in the human and canine intervertebral disc during maturation and degeneration.JOR spine · 2019Article
- NFAT5 and HIF-1α Coordinate to Regulate NKCC1 Expression in Hippocampal Neurons After Hypoxia-Ischemia.Frontiers in cell and developmental biology · 2019Article
- The potential of CRISPR/Cas9 genome editing for the study and treatment of intervertebral disc pathologies.JOR spine · 2018Review
- Hyperosmolarity induces notochordal cell differentiation with aquaporin3 upregulation and reduced N-cadherin expression.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2018Article
- Effects of Inflammation on Multiscale Biomechanical Properties of Cartilaginous Cells and Tissues.ACS biomaterials science & engineering · 2017Review
- Formation, function, and exhaustion of notochordal cytoplasmic vacuoles within intervertebral disc: current understanding and speculation.Oncotarget · 2017Review
- Article
- Expression of aquaporin1, a water channel protein, in cytoplasm is negatively correlated with prognosis of breast cancer patients.Oncotarget · 2016Article
- Disc in flames: Roles of TNF-α and IL-1β in intervertebral disc degeneration.European cells & materials · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
Objectives of this study were to investigate whether AQP1 and AQP5 expression is altered during intervertebral disc degeneration and if hypoxia and HIF-1 regulate their expression in NP cells. AQP expression was measured in human tissues from different degenerative grades; regulation by hypoxia and HIF-1 was studied using promoter analysis and gain- and loss-of-function experiments. We show that both AQPs are expressed in the disc and that mRNA and protein levels decline with human disease severity. Bioinformatic analyses of AQP promoters showed multiple evolutionarily conserved HREs. Surprisingly, hypoxia failed to induce promoter activity or expression of either AQP. While genomic chromatin immunoprecipitation showed limited binding of HIF-1α to conserved HREs, their mutation did not suppress promoter activities. Stable HIF-1α suppression significantly decreased mRNA and protein levels of both AQPs, but HIF-1α failed to induce AQP levels following accumulation. Together, our results demonstrate that AQP1 and AQP5 expression is sensitive to human disc degeneration and that HIF-1α uniquely maintains basal expression of both AQPs in NP cells, independent of oxemic tension and HIF-1 binding to promoter HREs. Diminished HIF-1 activity during degeneration may suppress AQP levels in NP cells, compromising their ability to respond to extracellular osmolarity changes.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.