ArticleCell reports2015
Individual and collective contributions of chaperoning and degradation to protein homeostasis in E. coli.
Article in Cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 45 citations in OpenAlex.
- Considering Metabolic Context in Enzyme Evolution and Design.Biochemistry · 2025Review
- From Rat Tails to Glycoproteostasis: Motivated by Biology, Enabled by Biophysics, and Lucky.Journal of molecular biology · 2025Review
- Novel Strategy for In Vitro Validation of Babesia orientalis Heat Shock Proteins Chaperone Activity and Thermostability.Acta parasitologica · 2024Article
- The ins and outs of Bacillus proteases: activities, functions and commercial significance.FEMS microbiology reviews · 2022Review
- Inorganic polyphosphate in host and microbe biology.Trends in microbiology · 2021Review
- The Proteome Folding Problem and Cellular Proteostasis.Journal of molecular biology · 2021Review
- Quantitative interrogation of protein co-aggregation using multi-color fluorogenic protein aggregation sensors.Chemical science · 2021Article
- 'Frontiers in microbiology · 2021Article
- Emerging Adaptive Strategies Under Temperature Fluctuations in a Laboratory Evolution Experiment ofFrontiers in microbiology · 2021Article
- Effect of Protein Structure on Evolution of Cotranslational Folding.Biophysical journal · 2020Article
- Article
- KLR-70: A Novel Cationic Inhibitor of the Bacterial Hsp70 Chaperone.Biochemistry · 2020Article
- Article
- Kinetic versus thermodynamic control of mutational effects on protein homeostasis: A perspective from computational modeling and experiment.Protein science : a publication of the Protein Society · 2019Article
- Protein-Protein Interactions in the Molecular Chaperone Network.Accounts of chemical research · 2018Review
- Thermosensitivity of growth is determined by chaperone-mediated proteome reallocation.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Functional characterization of LotP from Liberibacter asiaticus.Microbial biotechnology · 2017Article
- Ligand-promoted protein folding by biased kinetic partitioning.Nature chemical biology · 2017Article
- Bacterial proteostasis balances energy and chaperone utilization efficiently.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Bridging the physical scales in evolutionary biology: from protein sequence space to fitness of organisms and populations.Current opinion in structural biology · 2017Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
The folding fate of a protein in vivo is determined by the interplay between a protein's folding energy landscape and the actions of the proteostasis network, including molecular chaperones and degradation enzymes. The mechanisms of individual components of the E. coli proteostasis network have been studied extensively, but much less is known about how they function as a system. We used an integrated experimental and computational approach to quantitatively analyze the folding outcomes (native folding versus aggregation versus degradation) of three test proteins biosynthesized in E. coli under a variety of conditions. Overexpression of the entire proteostasis network benefited all three test proteins, but the effect of upregulating individual chaperones or the major degradation enzyme, Lon, varied for proteins with different biophysical properties. In sum, the impact of the E. coli proteostasis network is a consequence of concerted action by the Hsp70 system (DnaK/DnaJ/GrpE), the Hsp60 system (GroEL/GroES), and Lon.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.