ArticleThe Journal of surgical research2015
Intestinal alkaline phosphatase to treat necrotizing enterocolitis.
Article in The Journal of surgical research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Necrotizing enterocolitis: a potential protective role for intestinal alkaline phosphatase as lipopolysaccharide detoxifying enzyme.Frontiers in pediatrics · 2024Article
- Necrotizing enterocolitis in premature infants-A defect in the brakes? Evidence from clinical and animal studies.Mucosal immunology · 2023Review
- Protective Effect of Alkaline Phosphatase Supplementation on Infant Health.Foods (Basel, Switzerland) · 2022Review
- Emerging prediction methods for early diagnosis of necrotizing enterocolitis.Frontiers in medicine · 2022Review
- Targeting the Intestinal Barrier to Prevent Gut-Derived Inflammation and Disease: A Role for Intestinal Alkaline Phosphatase.Visceral medicine · 2021Review
- Influence of Human Milk on Very Preterms' Gut Microbiota and Alkaline Phosphatase Activity.Nutrients · 2021Observational
- Ontogeny of alkaline phosphatase activity in infant intestines and breast milk.BMC pediatrics · 2019Article
- Human ALPI deficiency causes inflammatory bowel disease and highlights a key mechanism of gut homeostasis.EMBO molecular medicine · 2018Article
- [Research advances in necrotizing enterocolitis in neonates].Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics · 2018Review
- Alkaline Phosphatase, an Unconventional Immune Protein.Frontiers in immunology · 2017Review
- Experimental Anti-Inflammatory Drug Semapimod Inhibits TLR Signaling by Targeting the TLR Chaperone gp96.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundIntestinal alkaline phosphatase (IAP) activity is decreased in necrotizing enterocolitis (NEC), and IAP supplementation prevents NEC development. It is not known if IAP given after NEC onset can reverse the course of the disease. We hypothesized that enteral IAP given after NEC induction would not reverse intestinal injury. MATERIALS AND
methodsNEC was induced in Sprague-Dawley pups by delivery preterm followed by formula feedings with lipopolysaccharide (LPS) and hypoxia exposure and continued up to 4 d. IAP was added to feeds on day 2 until being sacrificed on day 4. NEC severity was scored based on hematoxylin and eosin-stained terminal ileum sections, and AP activity was measured using a colorimetric assay. IAP and interleukin-6 expression were measured using real time polymerase chain reaction.
resultsNEC pups' alkaline phosphatase (AP) activity was decreased to 0.18 U/mg compared with controls of 0.57 U/mg (P < 0.01). Discontinuation of LPS and hypoxia after 2 d increased AP activity to 0.36 U/mg (P < 0.01). IAP supplementation in matched groups did not impact total AP activity or expression. Discontinuing LPS and hypoxia after NEC onset improved intestinal injury scores to 1.14 compared with continued stressors, score 2.25 (P < 0.01). IAP supplementation decreased interleukin-6 expression two-fold (P < 0.05), though did not reverse NEC intestinal damage (P = 0.5).
conclusionsThis is the first work to demonstrate that removing the source of NEC improves intestinal damage and increases AP activity. When used as a rescue treatment, IAP decreased intestinal inflammation though did not impact injury making it likely that IAP is best used preventatively to those neonates at risk.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.