ArticleJournal of industrial microbiology & biotechnology2015
Overexpression and characterization of a Ca(2+) activated thermostable β-glucosidase with high ginsenoside Rb1 to ginsenoside 20(S)-Rg3 bioconversion productivity.
Article in Journal of industrial microbiology & biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Efficient Biosynthesis of Gastrodin by UDP-Glycosyltransferase fromJournal of microbiology and biotechnology · 2025Article
- Rare ginsenosides: A unique perspective of ginseng research.Journal of advanced research · 2024Review
- Recombinant Expression and Characterization of an Alkali-tolerant UDP-Glycosyltransferase fromJournal of microbiology and biotechnology · 2024Article
- Recombinant GH3 β-glucosidase stimulated by xylose and tolerant to furfural and 5-hydroxymethylfurfural obtained from Aspergillus nidulans.Bioresources and bioprocessing · 2024Article
- Advancements in the Biotransformation and Biosynthesis of the Primary Active Flavonoids Derived fromMolecules (Basel, Switzerland) · 2023Review
- β-Glucosidase and Its Application in Bioconversion of Ginsenosides inBioengineering (Basel, Switzerland) · 2023Review
- Biotransformation of High Concentrations of Ginsenoside Substrate into Compound K by β-glycosidase fromGenes · 2023Article
- Progress in the Conversion of Ginsenoside Rb1 into Minor Ginsenosides Using β-Glucosidases.Foods (Basel, Switzerland) · 2023Review
- Characterization of a novel thermophilic beta-glucosidase from3 Biotech · 2022Article
- Cloning and characterization of thermophilic endoglucanase and its application in the transformation of ginsenosides.AMB Express · 2022Article
- Highly Efficient Biotransformation of Notoginsenoside R1 into Ginsenoside Rg1 byJournal of microbiology and biotechnology · 2022Article
- Study on the Biochemical Characterization and Selectivity of Three β-Glucosidases FromFrontiers in microbiology · 2022Article
- Immobilization of GH78 α-L-Rhamnosidase fromJournal of microbiology and biotechnology · 2021Article
- One-step purification of two novel thermotolerant β-1,4-glucosidases from a newly isolated strain of Fusarium chlamydosporum HML278 and their characterization.AMB Express · 2020Article
- Identification and molecular characterization of a psychrophilic GH1 β-glucosidase from the subtropical soil microorganism Exiguobacterium sp. GXG2.AMB Express · 2019Article
- Effects of β-glucosidase and α-rhamnosidase on the Contents of Flavonoids, Ginkgolides, and Aroma Components in Ginkgo Tea Drink.Molecules (Basel, Switzerland) · 2019Article
- Cellulases from Thermophiles Found by Metagenomics.Microorganisms · 2018Review
- Data on thermostable β-glucosidase immobilized by ZnData in brief · 2018Article
- Characterization of a novel thermostable and xylose-tolerant GH 39 β-xylosidase from Dictyoglomus thermophilum.BMC biotechnology · 2018Article
- Cloning and characterization of enoate reductase with high β-ionone to dihydro-β-ionone bioconversion productivity.BMC biotechnology · 2018Article
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Abstract
The thermostable β-glucosidase gene from Thermotoga petrophila DSM 13995 was cloned and overexpressed in Escherichia coli. The activity of the recombinant β-glucosidase was 21 U/mL in the LB medium. Recombinant β-glucosidase was purified, and its molecular weight was approximately 81 kDa. The optimal activity was at pH 5.0 and 90 °C, and the thermostability of the enzyme was improved by Ca(2+). The β-glucosidase had high selectivity for cleaving the outer and inner glucopyranosyl moieties at the C-20 carbon of ginsenoside Rb1, which produced the pharmacologically active minor ginsenoside 20(S)-Rg3. In a reaction at 90 °C and pH 5.0, 10 g/L of ginsenoside Rb1 was transformed into 6.93 g/L of Rg3 within 90 min, with a corresponding molar conversion of 97.9%, and Rg3 productivity of 4620 mg/L/h. This study is the first report of a GH3-family enzyme that used Ca(2+) to improve its thermostability, and it is the first report on the high substrate concentration bioconversion of ginsenoside Rb1 to ginsenoside 20(S)-Rg3 by using thermostable β-glucosidase under high temperature.
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