ArticleInternational journal of molecular sciences2015
Characterization and structure prediction of partial length protein sequences of pcoA, pcoR and chrB genes from heavy metal resistant bacteria from the Klip River, South Africa.
Article in International journal of molecular sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 39 citations in OpenAlex.
- Unravelling the mechanisms of antibiotic and heavy metal resistance co-selection in environmental bacteria.FEMS microbiology reviews · 2024Review
- A PadR family transcriptional repressor regulates the transcription of chromate efflux transporter in Enterobacter sp. Z1.Journal of microbiology (Seoul, Korea) · 2024Article
- First Description of Various Bacteria Resistant to Heavy Metals and Antibiotics Isolated from Polluted Sites in Tunisia.Polish journal of microbiology · 2021Article
- Article
- Genetic Determinants for Metal Tolerance and Antimicrobial Resistance Detected in Bacteria Isolated from Soils of Olive Tree Farms.Antibiotics (Basel, Switzerland) · 2020Article
- Chromate detoxification potential of Staphylococcus sp. isolates from an estuary.Ecotoxicology (London, England) · 2019Article
- Combined Transcriptome and Proteome Analysis of RpoS Regulon Reveals Its Role in Spoilage Potential ofFrontiers in microbiology · 2019Article
- Do environmentally induced DNA variations mediate adaptation in Aspergillus flavus exposed to chromium stress in tannery sludge?BMC genomics · 2018Article
- [Characterization of chromate resistance in genetically engineered Escherichia coli expressing chromate ion transporter ChrA].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2017Article
- Resistance to Antibiotics, Biocides, Preservatives and Metals in Bacteria Isolated from Seafoods: Co-Selection of Strains Resistant or Tolerant to Different Classes of Compounds.Frontiers in microbiology · 2017Article
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Authors and funding
3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The Klip River has suffered from severe anthropogenic effects from industrial activities such as mining. Long-term exposure to heavy metal pollution has led to the development of heavy metal resistant strains of Pseudomonas sp. KR23, Lysinibacillus sp. KR25, and E. coli KR29. The objectives of this study were to characterize the genetics of copper and chromate resistance of the isolates. Copper and chromate resistance determinants were cloned and sequenced. Open reading frames (ORFs) related to the genes CopA and CopR were identified in E. coli KR29, PcoA in Lysinibacillus sp. KR25 and none related to chromate resistance were detected. The 3D-models predicted by I-TASSER disclose that the PcoA proteins consist of β-sheets, which form a part of the cupredoxin domain of the CopA copper resistance family of genes. The model for PcoR_29 revealed the presence of a helix turn helix; this forms part of a DNA binding protein, which is part of a heavy metal transcriptional regulator. The bacterial strains were cured using ethidium bromide. The genes encoding for heavy metal resistance and antibiotic resistance were found to be located on the chromosome for both Pseudomonas sp. (KR23) and E. coli (KR29). For Lysinibacillus (KR25) the heavy metal resistance determinants are suspected to be located on a mobile genetic element, which was not detected using gel electrophoresis.
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