ArticlePloS one2015
Optimizing culture medium composition to improve oligodendrocyte progenitor cell yields in vitro from subventricular zone-derived neural progenitor cell neurospheres.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 12 citations in OpenAlex.
- Identifying Genes that Affect Differentiation of Human Neural Stem Cells and Myelination of Mature Oligodendrocytes.Cellular and molecular neurobiology · 2023Article
- Culture Protocol and Transcriptomic Analysis of Murine SVZ NPCs and OPCs.Stem cell reviews and reports · 2023Article
- Expression and function of PDGF-C in development and stem cells.Open biology · 2021Review
- Oligodendrocyte ARNT2 expression is altered in models of MS.Neurology(R) neuroimmunology & neuroinflammation · 2020Article
- N-Phenylquinazolin-2-amine Yhhu4952 as a novel promotor for oligodendrocyte differentiation and myelination.Scientific reports · 2018Article
- [Conditioned medium from rat RSC96 cells promotes proliferation of oligodendrocyte progenitor cells in vitro].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2017Article
- High Yield of Adult Oligodendrocyte Lineage Cells Obtained from Meningeal Biopsy.Frontiers in pharmacology · 2017Article
- Primary Cell Culture Systems for Human Thyroid Studies.Thyroid : official journal of the American Thyroid Association · 2016Article
- Co-culture of oligodendrocytes and neurons can be used to assess drugs for axon regeneration in the central nervous system.Neural regeneration research · 2015Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neural Stem and Progenitor Cells (NSC/NPC) are gathering tangible recognition for their uses in cell therapy and cell replacement therapies for human disease, as well as a model system to continue research on overall neural developmental processes in vitro. The Subventricular Zone is one of the largest NSC/NPC niches in the developing mammalian Central Nervous System, and persists through to adulthood. Oligodendrocyte progenitor cell (OPC) enriched cultures are usefull tools for in vitro studies as well as for cell replacement therapies for treating demyelination diseases. We used Subventricular Zone-derived NSC/NPC primary cultures from newborn mice and compared the effects of different growth factor combinations on cell proliferation and OPC yield. The Platelet Derived Growth Factor-AA and BB homodimers had a positive and significant impact on OPC generation. Furthermore, heparin addition to the culture media contributed to further increase overall culture yields. The OPC generated by this protocol were able to mature into Myelin Basic Protein-expressing cells and to interact with neurons in an in vitro co-culture system. As a whole, we describe an optimized in vitro method for increasing OPC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.