ArticleMethods in cell biology2015
Scanning and three-dimensional electron microscopy methods for the study of Trypanosoma brucei and Leishmania mexicana flagella.
Article in Methods in cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Temperature dependence of the motility of the parasitic microswimmer Trypanosoma brucei.Biophysical journal · 2026Article
- A scoping study of the whole-cell imaging literature as a foundation for the emerging field of cell anatomy.BMC biology · 2026Article
- Modelling motility of Trypanosoma brucei.PLoS computational biology · 2025Article
- A scoping study of the whole-cell imaging literature: a foundational corpus, potential for mesoscale data synthesis, and implications for standardization of an emerging field.bioRxiv : the preprint server for biology · 2025Article
- Proteins with proximal-distal asymmetries in axoneme localisation control flagellum beat frequency.Nature communications · 2025Article
- A phylogenetic profiling approach identifies novel ciliogenesis genes in Drosophila and C. elegans.The EMBO journal · 2023Article
- A short-term method to evaluate anti-leishmania drugs by inhibition of stage differentiation in Leishmania mexicana using flow cytometry.Experimental parasitology · 2023Article
- Article
- tREPs-A New Class of Functional tRNA-Encoded Peptides.ACS omega · 2022Article
- In-depth biological analysis of alteration in Plasmodium knowlesi-infected red blood cells using a noninvasive optical imaging technique.Parasites & vectors · 2022Article
- Deletion of Glutamine Synthetase Gene Disrupts the Survivability and Infectivity ofFrontiers in cellular and infection microbiology · 2021Article
- Essential roles for deubiquitination in Leishmania life cycle progression.PLoS pathogens · 2020Article
- Direction of flagellum beat propagation is controlled by proximal/distal outer dynein arm asymmetry.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Rab11 mediates selective recycling and endocytic trafficking in Trypanosoma brucei.Traffic (Copenhagen, Denmark) · 2018Article
- Let's get fISSical: fast in silico synchronization as a new tool for cell division cycle analysis.Parasitology · 2018Review
- Controlling transferrin receptor trafficking with GPI-valence in bloodstream stage African trypanosomes.PLoS pathogens · 2017Article
- Flagellar pocket restructuring through the Leishmania life cycle involves a discrete flagellum attachment zone.Journal of cell science · 2016Article
- Basal body multipotency and axonemal remodelling are two pathways to a 9+0 flagellum.Nature communications · 2015Article
- Two-headed outer- and inner-arm dyneins ofBiochemistry and biophysics reports · 2015Article
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4 authors.
Funding
Abstract
Three-dimensional electron microscopy tools have revolutionized our understanding of cell structure and molecular complexes in biology. Here, we describe methods for studying flagellar ultrastructure and biogenesis in two unicellular parasites-Trypanosoma brucei and Leishmania mexicana. We describe methods for the preparation of these parasites for scanning electron microscopy cellular electron tomography, and serial block face scanning electron microscopy (SBFSEM). These parasites have a highly ordered cell shape and form, with a defined positioning of internal cytoskeletal structures and organelles. We show how knowledge of these can be used to dissect cell cycles in both parasites and identify the old flagellum from the new in T. brucei. Finally, we demonstrate the use of SBFSEM three-dimensional models for analysis of individual whole cells, demonstrating the excellent potential this technique has for future studies of mutant cell lines.
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