ArticleMethods in cell biology2015
Total internal reflection fluorescence microscopy of intraflagellar transport in Tetrahymena thermophila.
Article in Methods in cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 24 citations in OpenAlex.
- Three-dimensional beating pattern of the ciliary tip in the live ciliate Tetrahymena.Journal of cell science · 2025Article
- Importin-9 and a TPR domain protein MpH drive periodic patterning of ciliary arrays in Tetrahymena.The Journal of cell biology · 2025Article
- Left-right cortical interactions drive intracellular pattern formation in the ciliate Tetrahymena.PLoS genetics · 2025Article
- The secretory pathway in Tetrahymena is organized for efficient constitutive secretion at ciliary pockets.iScience · 2024Article
- GnRH Induces Citrullination of the Cytoskeleton in Murine Gonadotrope Cells.International journal of molecular sciences · 2024Article
- Global and local functions of the Fused kinase ortholog CdaH in intracellular patterning in Tetrahymena.Journal of cell science · 2024Article
- CEP104/FAP256 and associated cap complex maintain stability of the ciliary tip.The Journal of cell biology · 2023Article
- A practical reference for studying meiosis in the model ciliateMarine life science & technology · 2022Review
- PCD Genes-From Patients to Model Organisms and Back to Humans.International journal of molecular sciences · 2022Review
- Live-cell fluorescence imaging of ciliary dynamics.Biophysics reports · 2021Article
- Mutual antagonism between Hippo signaling and cyclin E drives intracellular pattern formation.The Journal of cell biology · 2020Article
- LF4/MOK and a CDK-related kinase regulate the number and length of cilia in Tetrahymena.PLoS genetics · 2019Article
- Motile Cilia: Innovation and Insight From Ciliate Model Organisms.Frontiers in cell and developmental biology · 2019Review
- Proteins that control the geometry of microtubules at the ends of cilia.The Journal of cell biology · 2018Article
- Intraflagellar transport: mechanisms of motor action, cooperation, and cargo delivery.The FEBS journal · 2017Review
- Cell Cycle-Related Kinase (CCRK) regulates ciliogenesis and Hedgehog signaling in mice.PLoS genetics · 2017Article
- Article
- The Intraflagellar Transport Machinery.Cold Spring Harbor perspectives in biology · 2016Review
- Methods for Studying Movement of Molecules Within Cilia.Methods in molecular biology (Clifton, N.J.) · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Live imaging has become a powerful tool in studies of ciliary proteins. Tetrahymena thermophila is an established ciliated model with well-developed genetic and biochemical approaches, but its large size, complex shape, and the large number of short and overlapping cilia, have made live imaging of ciliary proteins challenging. Here we describe a method that combines paralysis of cilia by nickel ions and total internal reflection microscopy for live imaging of fluorescent proteins inside cilia of Tetrahymena. Using this method, we quantitatively documented the intraflagellar transport in Tetrahymena.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.