Evidence map›Paper›PMID 25837389›Full record

ArticleMethods in cell biology2015

In vivo investigation of cilia structure and function using Xenopus.

Eric R Brooks, John B Wallingford

Open access · greenAbstract read
In one paragraph

Article in Methods in cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
7.8field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 24 citations in OpenAlex.

  1. Article
  2. Article
  3. Formation and function of multiciliated cells.The Journal of cell biology · 2024
    Review
  4. Article
  5. Article
  6. PCD Genes-From Patients to Model Organisms and Back to Humans.International journal of molecular sciences · 2022
    Review
  7. Article
  8. Article
  9. A 'tad' of hope in the fight against airway disease.Biochemical Society transactions · 2020
    Review
  10. The Many Faces ofFrontiers in physiology · 2019
    Article
  11. Article
  12. Article
  13. Article
  14. Review
  15. Article
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Eric R BrooksDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, TX, United States.
John B WallingfordDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, TX, United States; The Howard Hughes Medical Institute, Chevy Chase, MD, USA.
The University of Texas at Austin · US

Funding

Development and function in mucociliary epitheliaR01HL117164 · NHLBI · UNIVERSITY OF TEXAS AT AUSTIN · PI John B Wallingford · 2013 to 2026
$4.7M
Mechanism of vertebrate neural tube morphogenesisR01GM074104 · NIGMS · UNIVERSITY OF TEXAS AUSTIN · PI WALLINGFORD, JOHN B · 2005 to 2014
$2.6M
Howard Hughes Medical InstituteNHLBI NIH HHS R01 HL117164NIGMS NIH HHS R01 GM074104
6 · The paper itself

Abstract

Cilia are key organelles in development and homeostasis. The ever-expanding complement of cilia associated proteins necessitates rapid and tractable models for in vivo functional investigation. Xenopus laevis provides an attractive model for such studies, having multiple ciliated populations, including primary and multiciliated tissues. The rapid external development of Xenopus and the large cells make it an especially excellent platform for imaging studies. Here we present embryological and cell biological methods for the investigation of cilia structure and function in X. laevis, with a focus on quantitative live and fixed imaging.

Indexed as

AnimalsAxonemeBasal BodiesCiliaEmbryo, NonmammalianFluorescent Antibody TechniqueImage Processing, Computer-AssistedMicroscopy, ConfocalMorpholinosOligonucleotides, AntisenseStaining and LabelingTissue FixationXenopus laevisMorpholinosOligonucleotides, AntisenseBasal bodiesCiliaConfocal microscopyIntraflagellar transportMulticiliated cellsQuantitative imagingXenopus

Identifiers

PMID25837389
PMCPMC4433029
OpenAlexW785899419

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.