ArticleThe Journal of biological chemistry2015
Spatial Control of Epsin-induced Clathrin Assembly by Membrane Curvature.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- Membrane Curvature and Cancer: Mechanisms, Implications, and Therapeutic Perspectives.Cancers · 2026Review
- Dynamic GLUT trafficking at high glucose levels enhances insulin secretion: Dysregulation leads to decreased insulin secretion during type 2 diabetes.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Identification of Key Genes Involved in Seed Germination ofInternational journal of molecular sciences · 2024Article
- Protein-Protein Interactions on Membrane Surfaces Analysed Using Pull-Downs with Supported Bilayers on Silica Beads.The Journal of membrane biology · 2022Article
- Structure of the endocytic adaptor complex reveals the basis for efficient membrane anchoring during clathrin-mediated endocytosis.Nature communications · 2021Article
- Complimentary action of structured and unstructured domains of epsin supports clathrin-mediated endocytosis at high tension.Communications biology · 2020Article
- Murine Epsins Play an Integral Role in Podocyte Function.Journal of the American Society of Nephrology : JASN · 2020Article
- Cargo regulates clathrin-coated pit invagination via clathrin light chain phosphorylation.The Journal of cell biology · 2018Article
- A novel fluorescence microscopic approach to quantitatively analyse protein-induced membrane remodelling.Journal of biosciences · 2018Review
- Cytosolic proteins can exploit membrane localization to trigger functional assembly.PLoS computational biology · 2018Article
- Membrane fission by protein crowding.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Use of the supported membrane tube assay system for real-time analysis of membrane fission reactions.Nature protocols · 2017Article
- Comparative analysis of adaptor-mediated clathrin assembly reveals general principles for adaptor clustering.Molecular biology of the cell · 2016Article
- Epsin2 promotes polarity establishment and meiotic division through activating Cdc42 in mouse oocyte.Oncotarget · 2016Article
- Clathrin binding by the adaptor Ent5 promotes late stages of clathrin coat maturation.Molecular biology of the cell · 2016Article
- Automated Multi-Peak Tracking Kymography (AMTraK): A Tool to Quantify Sub-Cellular Dynamics with Sub-Pixel Accuracy.PloS one · 2016Article
- Thomas Pucadyil: Piecing together membrane fission.The Journal of cell biology · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Epsins belong to the family of highly conserved clathrin-associated sorting proteins that are indispensable for clathrin-mediated endocytosis, but their precise functions remain unclear. We have developed an assay system of budded supported membrane tubes displaying planar and highly curved membrane surfaces to analyze intrinsic membrane curvature preference shown by clathrin-associated sorting proteins. Using real-time fluorescence microscopy, we find that epsin preferentially partitions to and assembles clathrin on highly curved membrane surfaces. Sorting of epsin to regions of high curvature strictly depends on binding to phosphatidylinositol 4,5-bisphosphate. Fluorescently labeled clathrins rapidly assemble as foci, which in turn cluster epsin, while maintaining tube integrity. Clathrin foci grow in intensity with a typical time constant of ∼75 s, similar to the time scales for coated pit formation seen in cells. Epsin therefore effectively senses membrane curvature to spatially control clathrin assembly. Our results highlight the potential role of membrane curvature in orchestrating the myriad molecular interactions necessary for the success of clathrin-mediated membrane budding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.