ArticleBiosensors2015
Label-free imaging and biochemical characterization of bovine sperm cells.
Article in Biosensors, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Non-invasive applications of Raman spectroscopy in assisted reproduction.Frontiers in endocrinology · 2025Review
- A narrative review of sperm selection technology for assisted reproduction techniques.Translational andrology and urology · 2024Review
- Predicting Boar Sperm Survival during Liquid Storage Using Vibrational Spectroscopic Techniques.Biology · 2024Article
- Correlative Raman Imaging: Development and Cancer Applications.Biosensors · 2024Review
- The History and Prospects of Rabbit Sperm Sexing.Veterinary sciences · 2023Review
- Cancer metabolic features allow discrimination of tumor from white blood cells by label-free multimodal optical imaging.Frontiers in bioengineering and biotechnology · 2023Article
- Human live spermatozoa morphology assessment using digital holographic microscopy.Scientific reports · 2022Article
- Label-Free Evaluation of Chromatin Condensation in Human Normal Morphology Sperm Using Raman Spectroscopy.Reproductive sciences (Thousand Oaks, Calif.) · 2021Article
- Application and Progress of Raman Spectroscopy in Male Reproductive System.Frontiers in cell and developmental biology · 2021Review
- Raman Microscopy: Progress in Research on Cancer Cell Sensing.Sensors (Basel, Switzerland) · 2020Review
- High spatially sensitive quantitative phase imaging assisted with deep neural network for classification of human spermatozoa under stressed condition.Scientific reports · 2020Article
- High-throughput sperm assay using label-free microscopy: morphometric comparison between different sperm structures of boar and stallion spermatozoa.Animal reproduction science · 2020Article
- Reconstruction of bovine spermatozoa substances distribution and morphological differences between Holstein and Korean native cattle using three-dimensional refractive index tomography.Scientific reports · 2019Article
- Combined Raman and polarization sensitive holographic imaging for a multimodal label-free assessment of human sperm function.Scientific reports · 2019Article
- Advances in Cryopreservation of Bull Sperm.Frontiers in veterinary science · 2019Review
- Resistance and Raman spectroscopy analysis of Parageobacillus thermantarcticus spores after γ-ray exposure.Extremophiles : life under extreme conditions · 2018Article
- 3D imaging of sex-sorted bovine spermatozoon locomotion, head spin and flagellum beating.Scientific reports · 2018Article
- Spectral features of nuclear DNA in human sperm assessed by Raman Microspectroscopy: Effects of UV-irradiation and hydration.PloS one · 2018Article
- Non-Invasive Approaches to Epigenetic-Based Sperm Selection.Medical science monitor : international medical journal of experimental and clinical research · 2017Review
- Modulated Raman Spectroscopy for Enhanced Cancer Diagnosis at the Cellular Level.Sensors (Basel, Switzerland) · 2015Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A full label-free morphological and biochemical characterization is desirable to select spermatozoa during preparation for artificial insemination. In order to study these fundamental parameters, we take advantage of two attractive techniques: digital holography (DH) and Raman spectroscopy (RS). DH presents new opportunities for studying morphological aspect of cells and tissues non-invasively, quantitatively and without the need for staining or tagging, while RS is a very specific technique allowing the biochemical analysis of cellular components with a spatial resolution in the sub-micrometer range. In this paper, morphological and biochemical bovine sperm cell alterations were studied using these techniques. In addition, a complementary DH and RS study was performed to identify X- and Y-chromosome-bearing sperm cells. We demonstrate that the two techniques together are a powerful and highly efficient tool elucidating some important criterions for sperm morphological selection and sex-identification, overcoming many of the limitations associated with existing protocols.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.