ArticleSensors (Basel, Switzerland)2015
Rationally designing aptamer sequences with reduced affinity for controlled sensor performance.
Article in Sensors (Basel, Switzerland), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 15 citations in OpenAlex.
- Retention-Based, Dissociation-Sensitive Pre-Screening of Aptamers Using a Centrifugal Microspin Filter.Biosensors · 2026Article
- Rational Approach to Optimizing Conformation-Switching Aptamers for Biosensing Applications.ACS sensors · 2024Article
- Microscale, Electrochemical, Aptamer-Based Sensors for Enhanced Small-Molecule Detection at Millisecond Time Scales.ACS sensors · 2023Article
- A capacitive laser-induced graphene based aptasensor for SARS-CoV-2 detection in human saliva.PloS one · 2023Article
- Programmable self-regulated molecular buffers for precise sustained drug delivery.Nature communications · 2022Article
- Rational Design of a User-Friendly Aptamer/Peptide-Based Device for the Detection ofSensors (Basel, Switzerland) · 2020Article
- Perspective on the Future Role of Aptamers in Analytical Chemistry.Analytical chemistry · 2019Article
- Electrochemical Studies of Cation Condensation-Induced Collapse of Surface-Bound DNA.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Reagentless, Structure-Switching, Electrochemical Aptamer-Based Sensors.Annual review of analytical chemistry (Palo Alto, Calif.) · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The relative ease of predicting the secondary structure of nucleic acid sequences lends itself to the design of sequences to perform desired functions. Here, we combine the utility of nucleic acid aptamers with predictable control over the secondary structure to rationally design sequences with controlled affinity towards a target analyte when employed as the recognition element in an electrochemical sensor. Specifically, we present a method to modify an existing high-gain aptamer sequence to create sequences that, when employed in an electrochemical, aptamer-based sensor, exhibit reduced affinity towards a small molecule analyte tobramycin. Sensors fabricated with the high-gain parent sequence saturate at concentrations much below the therapeutic window for tobramycin (7-18 µM). Accordingly, the rationale behind modifying this high-gain sequence to reduce binding affinity was to tune sensor performance for optimal sensitivity in the therapeutic window. Using secondary structure predictions and analysis of the NMR structure of an aminoglycoside RNA aptamer bound to tobramycin, we are able to successfully modify the aptamer sequence to tune the dissociation constants of electrochemical aptamer-based sensors between 0.17 and 3 µM. The guidelines we present represent a general strategy to lessening binding affinity of sensors employing aptamer-modified electrodes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.