ArticlePeerJ2015
Low-cost, low-input RNA-seq protocols perform nearly as well as high-input protocols.
Article in PeerJ, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- Article
- The Genome and mRNA Transcriptome of the Cosmopolitan Calanoid Copepod Acartia tonsa Dana Improve the Understanding of Copepod Genome Size Evolution.Genome biology and evolution · 2019Article
- Article
- Spatially varying cis-regulatory divergence in Drosophila embryos elucidates cis-regulatory logic.PLoS genetics · 2018Article
- Missing data and technical variability in single-cell RNA-sequencing experiments.Biostatistics (Oxford, England) · 2018Article
- A comparative analysis of library prep approaches for sequencing low input translatome samples.BMC genomics · 2018Article
- Cloud computing for genomic data analysis and collaboration.Nature reviews. Genetics · 2018Review
- Rail-RNA: scalable analysis of RNA-seq splicing and coverage.Bioinformatics (Oxford, England) · 2017Article
- mRNA pseudouridylation affects RNA metabolism in the parasiteRNA (New York, N.Y.) · 2017Article
- Single-cell RNA-sequencing: The future of genome biology is now.RNA biology · 2017Review
- Transcriptional characterization of Vibrio fischeri during colonization of juvenile Euprymna scolopes.Environmental microbiology · 2017Article
- Genome-wide measurement of spatial expression in patterning mutants ofF1000Research · 2017Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Recently, a number of protocols extending RNA-sequencing to the single-cell regime have been published. However, we were concerned that the additional steps to deal with such minute quantities of input sample would introduce serious biases that would make analysis of the data using existing approaches invalid. In this study, we performed a critical evaluation of several of these low-volume RNA-seq protocols, and found that they performed slightly less well in per-gene linearity of response, but with at least two orders of magnitude less sample required. We also explored a simple modification to one of these protocols that, for many samples, reduced the cost of library preparation to approximately $20/sample.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.