ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2015
A critical role for purinergic signalling in the mechanisms underlying generation of BOLD fMRI responses.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
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Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it, 57 citations in OpenAlex.
- CONature communications · 2022Pooled it
- ATP Metabolism of Astrocytes: Consumption, Regeneration and Restoration.Neurochemical research · 2025Review
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- Microglia modulate the cerebrovascular reactivity through ectonucleotidase CD39.Nature communications · 2025Article
- The role of neurovascular coupling dysfunction in cognitive decline of diabetes patients.Frontiers in neuroscience · 2024Review
- Neurovascular coupling mechanisms in health and neurovascular uncoupling in Alzheimer's disease.Brain : a journal of neurology · 2022Article
- Connexin and Pannexin Large-Pore Channels in Microcirculation and Neurovascular Coupling Function.International journal of molecular sciences · 2022Review
- Review
- Oxidative Stress and the Neurovascular Unit.Life (Basel, Switzerland) · 2021Review
- Age-related alterations in the cerebrovasculature affect neurovascular coupling and BOLD fMRI responses: Insights from animal models of aging.Psychophysiology · 2021Article
- Article
- Neurovascular Coupling in Development and Disease: Focus on Astrocytes.Frontiers in cell and developmental biology · 2021Review
- Astrocytes regulate brain extracellular pH via a neuronal activity-dependent bicarbonate shuttle.Nature communications · 2020Article
- Single-cell RNA sequencing identifies senescent cerebromicrovascular endothelial cells in the aged mouse brain.GeroScience · 2020Article
- Astrocytes monitor cerebral perfusion and control systemic circulation to maintain brain blood flow.Nature communications · 2020Article
- Electrochemical carbon fiber-based technique for simultaneous recordings of brain tissue POBiosensors & bioelectronics: X · 2019Article
- A critical role for the ATP-sensitive potassium channel subunit KJournal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2019Article
- Astrocyte dysfunction and neurovascular impairment in neurological disorders: Correlation or causation?Neurochemistry international · 2019Review
- Article
- Age- and Experience-Related Plasticity of ATP-Mediated Signaling in the Neocortex.Frontiers in cellular neuroscience · 2019Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
Abstract
The mechanisms of neurovascular coupling underlying generation of BOLD fMRI signals remain incompletely understood. It has been proposed that release of vasoactive substances by astrocytes couples neuronal activity to changes in cerebrovascular blood flow. However, the role of astrocytes in fMRI responses remains controversial. Astrocytes communicate via release of ATP, and here we tested the hypothesis that purinergic signaling plays a role in the mechanisms underlying fMRI. An established fMRI paradigm was used to trigger BOLD responses in the forepaw region of the somatosensory cortex (SSFP) of an anesthetized rat. Forepaw stimulation induced release of ATP in the SSFP region. To interfere with purinergic signaling by promoting rapid breakdown of the vesicular and/or released ATP, a lentiviral vector was used to express a potent ectonucleotidase, transmembrane prostatic acid phosphatase (TMPAP), in the SSFP region. TMPAP expression had no effect on resting cerebral blood flow, cerebrovascular reactivity, and neuronal responses to sensory stimulation. However, TMPAP catalytic activity markedly reduced the magnitude of BOLD fMRI responses triggered in the SSFP region by forepaw stimulation. Facilitated ATP breakdown could result in accumulation of adenosine. However, blockade of A1 receptors had no effect on BOLD responses and did not reverse the effect of TMPAP. These results suggest that purinergic signaling plays a significant role in generation of BOLD fMRI signals. We hypothesize that astrocytes activated during periods of enhanced neuronal activity release ATP, which propagates astrocytic activation, stimulates release of vasoactive substances and dilation of cerebral vasculature.
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